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首页> 外文期刊>Journal of bacteriology >Salicylic Acid, Yersiniabactin, and Pyoverdin Production by the Model Phytopathogen Pseudomonas syringae pv. tomato DC3000: Synthesis, Regulation, and Impact on Tomato and Arabidopsis Host Plants
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Salicylic Acid, Yersiniabactin, and Pyoverdin Production by the Model Phytopathogen Pseudomonas syringae pv. tomato DC3000: Synthesis, Regulation, and Impact on Tomato and Arabidopsis Host Plants

机译：水杨酸，耶尔西菌素和Pyoverdin由模型致病菌Pseudomonas syringae pv生产。番茄DC3000：合成，调控及其对番茄和拟南芥寄主植物的影响

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A genetically tractable model plant pathosystem, Pseudomonas syringae pv. tomato DC3000 on tomato and Arabidopsis thaliana hosts, was used to investigate the role of salicylic acid (SA) and iron acquisition via siderophores in bacterial virulence. Pathogen-induced SA accumulation mediates defense in these plants, and DC3000 contains the genes required for the synthesis of SA, the SA-incorporated siderophore yersiniabactin (Ybt), and the fluorescent siderophore pyoverdin (Pvd). We found that DC3000 synthesizes SA, Ybt, and Pvd under iron-limiting conditions in culture. Synthesis of SA and Ybt by DC3000 requires pchA, an isochorismate synthase gene in the Ybt genomic cluster, and exogenous SA can restore Ybt production by the pchA mutant. Ybt was also produced by DC3000 in planta, suggesting that Ybt plays a role in DC3000 pathogenesis. However, the pchA mutant did not exhibit any growth defect or altered virulence in plants. This lack of phenotype was not attributable to plant-produced SA restoring Ybt production, as the pchA mutant grew similarly to DC3000 in an Arabidopsis SA biosynthetic mutant, and in planta Ybt was not detected in pchA-infected wild-type plants. In culture, no growth defect was observed for the pchA mutant versus DC3000 for any condition tested. Instead, enhanced growth of the pchA mutant was observed under stringent iron limitation and additional stresses. This suggests that SA and Ybt production by DC3000 is costly and that Pvd is sufficient for iron acquisition. Further exploration of the comparative synthesis and utility of Ybt versus Pvd production by DC3000 found siderophore-dependent amplification of ybt gene expression to be absent, suggesting that Ybt may play a yet unknown role in DC3000 pathogenesis.
机译：一种遗传易处理的模型植物病理系统，丁香假单胞菌 pv。番茄和拟南芥宿主上的番茄DC3000用于研究水杨酸（SA）和通过铁载体获得铁在细菌毒力中的作用。病原体诱导的SA积累介导了这些植物的防御，DC3000包含合成SA，SA结合的铁载体耶尔西菌素（Ybt）和荧光铁载体pyoverdin（Pvd）所需的基因。我们发现DC3000在培养中的铁限制条件下可以合成SA，Ybt和Pvd。 DC3000合成SA和Ybt需要Ybt基因组簇中的等位基因合成酶 pchA ，外源SA可以恢复 pchA 突变体产生的Ybt。 Ybt也由DC3000在植物中产生，表明Ybt在DC3000发病机理中起作用。但是， pchA 突变体在植物中没有表现出任何生长缺陷或改变的毒力。表型的缺乏并非归因于植物产生的SA恢复Ybt的产生，因为 pchA 突变体在 Arabidopsis SA生物合成突变体中的生长与DC3000相似，而植物中的Ybt为在 pchA 感染的野生型植物中未检测到。在培养中，在任何测试条件下， pchA 突变体与DC3000相比均未观察到生长缺陷。相反，在严格的铁限制和附加胁迫下观察到了 pchA 突变体的生长增强。这表明DC3000生产SA和Ybt的成本很高，Pvd足以用于铁的获取。 DC3000对Ybt与Pvd产生的比较合成和实用性的进一步探索发现 ybt 基因表达的铁载体依赖性扩增缺失，这表明Ybt在DC3000发病机理中可能仍发挥未知的作用。

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