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首页> 外文期刊>Journal of bacteriology >Maintaining the Transcription Factor SpoIIID Level Late during Sporulation Causes Spore Defects in Bacillus subtilis
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Maintaining the Transcription Factor SpoIIID Level Late during Sporulation Causes Spore Defects in Bacillus subtilis

机译：孢子形成过程中后期保持转录因子SpoIIID水平会导致枯草芽孢杆菌中的孢子缺陷

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During sporulation of Bacillus subtilis, four regulatory proteins act in the order σE, SpoIIID, σK, and GerE to temporally control gene expression in the mother cell. σE and σK work sequentially with core RNA polymerase to transcribe different sets of genes. SpoIIID and GerE are small, sequence-specific DNA-binding proteins that activate or repress transcription of many genes. Previous studies showed that transcriptionally active σK RNA polymerase inhibits early mother cell gene expression, reducing accumulation of SpoIIID late in sporulation. Here, the effects of perturbing the mother cell gene regulatory network by maintaining the SpoIIID level late during sporulation are reported. Persistent expression was obtained by fusing spoIIID to the σK-controlled gerE promoter on a multicopy plasmid. Fewer heat- and lysozyme-resistant spores were produced by the strain with persistent spoIIID expression, but the number of spores resistant to organic solvents was unchanged, as was their germination ability. Transmission electron microscopy showed structural defects in the spore coat. Reporter fusions to σK-dependent promoters showed lower expression of gerE and cotC and higher expression of cotD. Altered expression of cot genes, which encode spore coat proteins, may account for the spore structural defects. These results suggest that one role of negative feedback by σK RNA polymerase on early mother cell gene expression is to lower the level of SpoIIID late during sporulation in order to allow normal expression of genes in the σK regulon.
机译：在枯草芽孢杆菌的孢子形成过程中，四种调节蛋白以σ E ，SpoIIID，σ K 和GerE的顺序发挥作用，以暂时控制大肠杆菌中的基因表达。母细胞。 σ E 和σ K 与核心RNA聚合酶顺序工作，以转录不同的基因集。 SpoIIID和GerE是小的，序列特异性的DNA结合蛋白，可以激活或抑制许多基因的转录。先前的研究表明，转录活性σ K RNA聚合酶可抑制早期母细胞基因表达，减少孢子形成后期SpoIIID的积累。在这里，报告了在孢子形成后期通过维持SpoIIID水平来干扰母细胞基因调控网络的影响。通过将 spoIIID 与多拷贝质粒上的σ K 控制的 gerE 启动子融合，可以获得持久的表达。持久表达 spoIIID 的菌株产生的耐热和耐溶菌酶的孢子较少，但是耐有机溶剂的孢子数量和发芽能力没有变化。透射电子显微镜显示孢子皮中的结构缺陷。记者与σ K 依赖的启动子融合后， gerE 和 cotC 的表达较低，而 cotD 的表达较高。编码孢子外壳蛋白的 cot 基因的表达改变可能是造成孢子结构缺陷的原因。这些结果表明，σ K RNA聚合酶对早期母细胞基因表达的负反馈作用之一是降低孢子形成后期的SpoIIID水平，以使σ中的基因正常表达。 > K 规则。

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