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首页> 外文期刊>Journal of bacteriology >Molecular Characterization of a Clostridium difficile Bacteriophage and Its Cloned Biologically Active Endolysin
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Molecular Characterization of a Clostridium difficile Bacteriophage and Its Cloned Biologically Active Endolysin

机译：艰难梭菌噬菌体的分子表征及其克隆的生物活性内溶素

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Clostridium difficile infection is increasing in both frequency and severity, with the emergence of new highly virulent strains highlighting the need for more rapid and effective methods of control. Here, we show that bacteriophage endolysin can be used to inhibit and kill C. difficile. The genome sequence of a novel bacteriophage that is active against C. difficile was determined, and the bacteriophage endolysin gene was subcloned and expressed in Escherichia coli. The partially purified endolysin was active against 30 diverse strains of C. difficile, and importantly, this group included strains of the major epidemic ribotype 027 (B1/NAP1). In contrast, a range of commensal species that inhabit the gastrointestinal tract, including several representatives of the clostridium-like Firmicutes, were insensitive to the endolysin. This endolysin provides a platform for the generation of both therapeutic and detection systems to combat the C. difficile problem. To investigate a method for the protected delivery and production of the lysin in the gastrointestinal tract, we demonstrated the expression of active CD27L endolysin in the lactic acid bacterium Lactococcus lactis MG1363.
机译：难辨梭状芽胞杆菌感染的频率和严重性都在增加，新的高毒力菌株的出现凸显了对更快速有效控制方法的需求。在这里，我们表明噬菌体内溶素可用于抑制和杀死C。困难。一种新型细菌噬菌体的基因组序列，对 C具有活性。确定艰难梭菌，并在大肠杆菌中亚克隆噬菌体内溶素基因。部分纯化的溶血素对30种不同的 C菌株具有活性。困难，而且重要的是，该组包括主要流行核糖体型027（B1 / NAP1）的菌株。相反，居住在胃肠道中的多种共生物种，包括梭状芽孢杆菌 Firmicutes 的几个代表，对内溶素不敏感。该细胞内溶素为对抗 C的治疗和检测系统的产生提供了平台。困难问题。为了研究在胃肠道中赖氨酸的保护性递送和生产的方法，我们证明了活性细菌CD27L内溶素在乳酸菌乳酸乳球菌MG1363中的表达。

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