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首页> 外文期刊>Journal of bacteriology >Growth Phase and Metal-Dependent Transcriptional Regulation of the fecA Genes in Helicobacter pylori
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Growth Phase and Metal-Dependent Transcriptional Regulation of the fecA Genes in Helicobacter pylori

机译：幽门螺杆菌中fecA基因的生长期和金属依赖性转录调控

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Balancing metal uptake is essential for maintaining a proper intracellular metal concentration. Here, we report the transcriptional control exerted by the two metal-responsive regulators of Helicobacter pylori, Fur (iron-dependent ferric uptake regulator) and NikR (nickel-responsive regulator), on the three copies of the fecA genes present in this species. By monitoring the patterns of transcription throughout growth and in response to nickel, iron, and a metal chelator, we found that the expression of the three fecA genes is temporally regulated, responds to metals in different ways, and is selectively controlled by either one of the two regulators. fecA1 is expressed at a constant level throughout growth, and its expression is iron sensitive; the expression of fecA2 is mainly off, with minor expression coming up in late exponential phase. In contrast, the expression of fecA3 is maximal in early exponential phase, gradually decreases with time, and is repressed by nickel. The direct roles of Fur and NikR were studied both in vitro, by mapping the binding sites of each regulator on the promoter regions via DNase I footprinting analysis, and in vivo, by using primer extension analyses of the fecA transcripts in fur and nikR deletion strains. Overall, the results show that the expression of each fecA gene is finely tuned in response to metal availability, as well as during the bacterial growth phase, suggesting specific and dedicated functions for the three distinct FecA homologues.
机译：平衡金属摄取对于维持适当的细胞内金属浓度至关重要。在这里，我们报道了幽门螺杆菌的两个金属响应性调节剂Fur（铁依赖性铁摄取调节剂）和NikR（镍响应性调节剂）对三个拷贝的转录控制。该物种中存在 fecA 基因。通过监测整个生长过程中转录的模式以及对镍，铁和金属螯合剂的反应，我们发现三个 fecA 基因的表达受到时间调节，以不同的方式对金属做出反应，并且由两个调节器之一有选择地控制。在整个生长过程中， fecA1 均以恒定水平表达，并且其表达对铁敏感; fecA2 的表达主要是关闭的，次要表达出现在指数后期。相反， fecA3 的表达在指数初期是最大的，随时间逐渐降低，并被镍抑制。通过DNase I足迹分析在启动子区域定位每个调节子的结合位点，在体外研究了Fur和NikR的直接作用;在体内，通过使用 fecA 的引物延伸分析，研究了Fur和NikR的直接作用。 fur 和 nikR 缺失菌株中的转录本。总体而言，结果表明，每个 fecA 基因的表达均根据金属的可利用性以及细菌的生长期进行了微调，表明这三种不同的FecA同源物具有特定的功能。

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