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首页> 外文期刊>Journal of bacteriology >Activity and Transcriptional Regulation of Bacterial Protein-Like Glycerol-3-Phosphate Dehydrogenase of the Haloarchaea in Haloferax volcanii
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Activity and Transcriptional Regulation of Bacterial Protein-Like Glycerol-3-Phosphate Dehydrogenase of the Haloarchaea in Haloferax volcanii

机译：火山嗜盐菌中类似细菌细菌性甘油3-磷酸甘油脱氢酶的活性和转录调控

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Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of “salty” biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conversion of G3P to dihydroxyacetone phosphate by G3P dehydrogenase (G3PDH). H. volcaniiharbors two putative G3PDH operons: (i) glpA1B1C1, located on the chromosome within the neighborhood of glpK, and (ii) glpA2B2C2, on megaplasmid pHV4. Analysis of knockout strains revealed that glpA1(and not glpA2) is required for growth on glycerol. However, both glpA1and glpA2could complement a glpA1knockout strain (when expressed from a strong promoter in trans) and were required for the total G3PDH activity of cell lysates. The glpA1B1C1, glpK, glpF(encoding a putative glycerol facilitator), and ptsH2(encoding a homolog of the bacterial phosphotransferase system protein Hpr) genes were transcriptionally linked and appeared to be under the control of a strong, G3P-inducible promoter upstream of glpA1. Overall, this study provides fundamental insights into glycerol metabolism in H. volcaniiand enhances our understanding of central metabolic pathways of haloarchaea.
机译：甘油是异养卤古菌的主要能源，也是“咸”生物柴油废物的主要成分。甘油仅由甘油激酶（由 glpK 编码）分解为Volofii Haloferax中的3-磷酸甘油（G3P）。在这里，我们表征了该代谢途径的下一个关键步骤：通过G3P脱氢酶（G3PDH）将G3P转化为磷酸二羟基丙酮。火山嗜血杆菌具有两个假定的G3PDH操纵子：（i） glpA1B1C1 ，位于 glpK 附近的染色体上，和（ii） glpA2B2C2 ，在大质粒pHV4上。敲除菌株的分析表明， glpA1 （而不是 glpA2 ）是在甘油上生长所必需的。但是， glpA1 和 glpA2 都可以补充 glpA1 敲除菌株（当在 trans 中由强启动子表达时）并且是细胞溶解产物总G3PDH活性所必需的。 glpA1B1C1 ， glpK ， glpF （编码假定的甘油促进剂）和 ptsH2 （编码细菌磷酸转移酶系统蛋白Hpr）基因被转录连接，并且似乎受 glpA1 上游一个强的，G3P诱导型启动子的控制。总的来说，这项研究提供了有关火山嗜酸菌甘油代谢的基本见识，并增进了我们对卤古菌中央代谢途径的了解。

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