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首页> 外文期刊>Journal of bacteriology >The Opportunistic Pathogen Serratia marcescens Utilizes Type VI Secretion To Target Bacterial Competitors
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The Opportunistic Pathogen Serratia marcescens Utilizes Type VI Secretion To Target Bacterial Competitors

机译：机会性致病性粘质沙雷氏菌利用VI型分泌物靶向细菌竞争者

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The type VI secretion system (T6SS) is the most recently described and least understood of the protein secretion systems of Gram-negative bacteria. It is widely distributed and has been implicated in the virulence of various pathogens, but its mechanism and exact mode of action remain to be defined. Additionally there have been several very recent reports that some T6SSs can target bacteria rather than eukaryotic cells. Serratia marcescens is an opportunistic enteric pathogen, a class of bacteria responsible for a significant proportion of hospital-acquired infections. We describe the identification of a functional T6SS in S. marcescens strain Db10, the first report of type VI secretion by an opportunist enteric bacterium. The T6SS of S. marcescens Db10 is active, with secretion of Hcp to the culture medium readily detected, and is expressed constitutively under normal growth conditions from a large transcriptional unit. Expression of the T6SS genes did not appear to be dependent on the integrity of the T6SS. The S. marcescens Db10 T6SS is not required for virulence in three nonmammalian virulence models. It does, however, exhibit dramatic antibacterial killing activity against several other bacterial species and is required for S. marcescens to persist in a mixed culture with another opportunist pathogen, Enterobacter cloacae. Importantly, this antibacterial killing activity is highly strain specific, with the S. marcescens Db10 T6SS being highly effective against another strain of S. marcescens with a very similar and active T6SS. We conclude that type VI secretion plays a crucial role in the competitiveness, and thus indirectly the virulence, of S. marcescens and other opportunistic bacterial pathogens.
机译：VI型分泌系统（T6SS）是革兰氏阴性细菌的蛋白质分泌系统的最新描述和最少了解。它广泛分布，并与各种病原体的毒力有关，但其作用机理和确切作用方式仍有待确定。另外，最近有一些报道称某些T6SS可以靶向细菌而不是真核细胞。粘质沙雷氏菌是一种机会性肠病原体，是一类细菌，可导致很大比例的医院获得性感染。我们描述了在marcescens菌株Db10中的功能性T6SS的鉴定，机会主义肠细菌的VI型分泌的第一个报道。 marcescens Db10的T6SS具有活性，Hcp向培养基的分泌很容易检测到，并在正常生长条件下从大的转录单位组成性表达。 T6SS基因的表达似乎并不依赖于T6SS的完整性。在三种非哺乳动物毒力模型中，不需要毒力链球菌Db10 T6SS。但是，它确实表现出了对其他几种细菌的显着抗菌杀灭活性，并且是marcescens与其他机会病原体阴沟肠杆菌混合培养所必需的。重要的是，这种抗菌杀伤活性是高度特异的菌株，marcescences Db10 T6SS对另一种具有非常相似和活性的T6SS的marcescens菌株非常有效。我们得出结论，VI型分泌物在粘液链球菌和其他机会性细菌病原体的竞争力中具有至关重要的作用，从而间接地提高了其毒性。

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