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首页> 外文期刊>Journal of bacteriology >Escherichia coli Insertion Sequence IS150: Transposition via Circular and Linear Intermediates
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Escherichia coli Insertion Sequence IS150: Transposition via Circular and Linear Intermediates

机译：大肠杆菌IS150插入序列：通过圆形和线性中间体进行转座

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IS150, a member of the widespread IS3 family, contains two consecutive out-of-phase open reading frames, orfA and orfB, that partially overlap. These open reading frames encode three proteins, InsA, InsB, and the InsAB protein, which is jointly encoded by both open reading frames by means of programmed translational frameshifting. We demonstrate that the InsAB protein represents the IS150 element's transposase. In vivo, the wild-type IS150 element generates circular excision products and linear IS150 molecules. Circular and linear species have previously been detected with mutant derivatives of other members of the IS3 family. Our finding supports the assumption that these products represent true transposition intermediates of members of this family. Analysis of the molecular nature of these two species suggested that the circular forms are precursors of the linear molecules. Elimination of InsA synthesis within the otherwise intact element led to accumulation of large amounts of the linear species, indicating that the primary role of InsA may be to prevent abortive production of the linear species and to couple generation of these species to productive insertion events.
机译：IS 150 是广泛使用的IS 3 家族的成员，包含两个连续的异相开放阅读框 orfA 和 orfB ，部分重叠。这些开放阅读框编码三种蛋白质，InsA，InsB和InsAB蛋白，这两个开放阅读框均通过程序化翻译移码共同编码。我们证明InsAB蛋白代表IS 150 元件的转座酶。在体内，野生型IS 150 元件可产生环状切除产物和线性IS 150 分子。先前已使用IS 3 家族其他成员的突变衍生物检测到了圆形和线性物种。我们的发现支持以下假设：这些产品代表该家族成员的真正转座中间体。对这两种物种的分子性质的分析表明，圆形是线性分子的前体。在原本完整的元件中消除InsA合成会导致大量线性物种的积聚，这表明InsA的主要作用可能是防止线性物种的流产，并将这些物种的产生与生产性插入事件联系起来。

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