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首页> 外文期刊>Journal of bacteriology >Characterization of Nutrient-Induced Dispersion in Pseudomonas aeruginosa PAO1 Biofilm
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Characterization of Nutrient-Induced Dispersion in Pseudomonas aeruginosa PAO1 Biofilm

机译：铜绿假单胞菌PAO1生物膜中营养诱导的分散体的表征

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The processes associated with early events in biofilm formation have become a major research focus over the past several years. Events associated with dispersion of cells from late stage biofilms have, however, received little attention. We demonstrate here that dispersal of Pseudomonas aeruginosa PAO1 from biofilms is inducible by a sudden increase in carbon substrate availability. Most efficient at inducing dispersal were sudden increases in availability of succinate > glutamate > glucose that led to ～80% reductions in surface-associated biofilm biomass. Nutrient-induced biofilm dispersion was associated with increased expression of flagella (fliC) and correspondingly decreased expression of pilus (pilA) genes in dispersed cells. Changes in gene expression associated with dispersion of P. aeruginosa biofilms were studied by using DNA microarray technology. Results corroborated proteomic data that showed gene expression to be markedly different between biofilms and newly dispersed cells. Gene families that were upregulated in dispersed cells included those for flagellar and ribosomal proteins, kinases, and phage PF1. Within the biofilm, genes encoding a number of denitrification pathways and pilus biosynthesis were also upregulated. Interestingly, nutrient-induced dispersion was associated with an increase in the number of Ser/Thr-phosphorylated proteins within the newly dispersed cells, and inhibition of dephosphorylation reduced the extent of nutrient-induced dispersion. This study is the first to demonstrate that dispersal of P. aeruginosa from biofilms can be induced by the addition of simple carbon sources. This study is also the first to demonstrate that dispersal of P. aeruginosa correlates with a specific dispersal phenotype.
机译：在过去的几年中，与生物膜形成的早期事件相关的过程已成为主要的研究重点。然而，与来自后期生物膜的细胞分散相关的事件很少受到关注。我们在这里证明了生物膜中铜绿假单胞菌PAO1的扩散是由碳底物可用性的突然增加诱导的。诱导分散最有效的是琥珀酸>谷氨酸>葡萄糖的利用率突然增加，这导致与表面相关的生物膜生物量减少了约80％。营养物诱导的生物膜分散与鞭毛（ fliC ）表达的增加和菌毛（ pilA ）基因在分散细胞中的表达减少有关。与 P分散有关的基因表达变化。利用DNA芯片技术研究了铜绿生物膜。结果证实了蛋白质组学数据，该数据表明基因表达在生物膜和新分散的细胞之间明显不同。在分散细胞中上调的基因家族包括鞭毛和核糖体蛋白，激酶和噬菌体PF1的那些。在生物膜内，编码许多反硝化途径和菌毛生物合成的基因也被上调。有趣的是，养分诱导的分散与新分散的细胞内Ser / Thr磷酸化蛋白数量的增加有关，去磷酸化的抑制降低了养分诱导的分散程度。这项研究是第一个证明 P扩散的研究。通过添加简单的碳源可以诱导生物膜中的铜绿菌。这项研究也是第一个证明 P分散的研究。铜绿假单胞菌与特定的扩散表型有关。

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