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首页> 外文期刊>Journal of bacteriology >Mapping of the Cryptococcus neoformans MATα Locus: Presence of Mating Type-Specific Mitogen-Activated Protein Kinase Cascade Homologs
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Mapping of the Cryptococcus neoformans MATα Locus: Presence of Mating Type-Specific Mitogen-Activated Protein Kinase Cascade Homologs

机译：新型隐球菌MATα基因座的映射：交配类型特定的丝裂原激活蛋白激酶级联同源的存在。

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In this study we investigated the relationship between theMATα locus of Cryptococcus neoformans and several MATα-specific mitogen-activated protein (MAP) kinase signal transduction cascade genes, includingSTE12α, STE11α, and STE20α. To resolve the location of the genes, we screened a cosmid library of the MATα strain B-4500 (JEC21), which was chosen for theC. neoformans genome project. We isolated several overlapping cosmids spanning a region of about 71 kb covering the entire MATα locus. It was found thatSTE12α, STE11α, and STE20α are imbedded within the locus rather than closely linked to the locus. Furthermore, three copies of MFα, the mating type α-pheromone gene, a MATα-specific myosin gene, and a pheromone receptor (CPRα) were identified within the locus. We created a physical map, based on the restriction enzymeBamHI, and identified both borders of theMATα locus. The MATα locus of C. neoformans is approximately 50 kb in size and is one of the largest mating type loci reported among fungi with a one-locus, two-allele mating system.
机译：在这项研究中，我们调查了隐球菌的 MAT α基因座与几种 MAT α特异性丝裂原激活蛋白（MAP）激酶之间的关系。信号转导级联基因，包括 STE12 α， STE11 α和 STE20 α。为了解析基因的位置，我们筛选了 MAT α菌株B-4500（JEC21）的粘粒文库，该文库用于 C。 Neoformans 基因组计划。我们分离了覆盖整个 MAT α基因座的大约71 kb区域的几个重叠粘粒。发现 STE12 α， STE11 α和 STE20 α嵌入在位点内，而不是紧密相连。此外，复制了三份 MF α，交配型α-信息素基因， MAT α特异性肌球蛋白基因和信息素受体（ CPR α）在基因座内被鉴定。我们基于限制酶 Bam HI创建了一个物理图谱，并确定了 MAT α基因座的两个边界。 C的 MAT α基因座。新甲虫的大小约为50 kb，是真菌中报告的最大的交配型基因座之一，具有单基因座，两等位基因的交配系统。

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