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首页> 外文期刊>Journal of bacteriology >Effect of PII and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System inKlebsiella pneumoniae
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Effect of PII and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System inKlebsiella pneumoniae

机译：PII及其同源GlnK通过异源表达红螺螺旋藻双氮酶还原酶ADP-核糖基转移酶-二氮酶还原酶激活的糖水解酶调节系统对肺炎克雷伯菌的可逆性ADP-二氮酶还原酶核糖基化的影响

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摘要

Reversible ADP-ribosylation of dinitrogenase reductase, catalyzed by the dinitrogenase reductase ADP-ribosyl transferase–dinitrogenase reductase-activating glycohydrolase (DRAT-DRAG) regulatory system, has been characterized in Rhodospirillum rubrum and other nitrogen-fixing bacteria. To investigate the mechanisms for the regulation of DRAT and DRAG activities, we studied the heterologous expression of R. rubrum draTG in Klebsiella pneumoniae glnB and glnK mutants. In K. pneumoniae wild type, the regulation of both DRAT and DRAG activity appears to be comparable to that seen in R. rubrum. However, the regulation of both DRAT and DRAG activities is altered in a glnB background. Some DRAT escapes regulation and becomes active under N-limiting conditions. The regulation of DRAG activity is also altered in a glnBmutant, with DRAG being inactivated more slowly in response to NH4 + treatment than is seen in wild type, resulting in a high residual nitrogenase activity. In aglnK background, the regulation of DRAT activity is similar to that seen in wild type. However, the regulation of DRAG activity is completely abolished in the glnK mutant; DRAG remains active even after NH4 + addition, so there is no loss of nitrogenase activity. The results with this heterologous expression system have implications for DRAT-DRAG regulation inR. rubrum.
机译：二氮酶还原酶ADP-核糖基转移酶-二氮酶还原酶激活糖水解酶（DRAT-DRAG）调节系统催化的可逆性ADP-核糖基化反应已被红螺螺旋藻和其他固氮细菌表征。为了研究调节DRAT和DRAG活性的机制，我们研究了 R的异源表达。 肺炎克雷伯菌glnB 和 glnK 突变体中的风疹draTG 。在 K。肺炎野生型中，DRAT和DRAG活性的调节似乎与 R中的相似。风疹。但是，在 glnB 背景下，DRAT和DRAG活动的调节均发生了变化。一些DRAT逃脱了调节，并在N极限条件下变得活跃。在 glnB 突变体中，DRAG活性的调节也发生了变化，与NH 4 + 处理相比，DRAG的失活更慢在野生型中，导致较高的残留固氮酶活性。在 glnK 背景下，DRAT活性的调节与野生型相似。但是， glnK 突变体中DRAG活性的调控被完全废除。即使添加了NH 4 + ，DRAG仍然保持活性，因此不会损失固氮酶的活性。该异源表达系统的结果对 R中的DRAT-DRAG调节具有影响。风疹。

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