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首页> 外文期刊>Journal of bacteriology >Codenitrification and Denitrification Are Dual Metabolic Pathways through Which Dinitrogen Evolves from Nitrate in Streptomyces antibioticus
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Codenitrification and Denitrification Are Dual Metabolic Pathways through Which Dinitrogen Evolves from Nitrate in Streptomyces antibioticus

机译：共硝化和反硝化是抗生素代谢链霉菌中的硝酸盐从硝酸盐演变成的双重代谢途径。

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We screened actinomycete strains for dinitrogen (N2)-producing activity and discovered that Streptomyces antibioticus B-546 evolves N2 and some nitrous oxide (N2O) from nitrate (NO3?). Most of the N2 that evolved from the heavy isotope ([15N]NO3?) was 15N14N, indicating that this nitrogen species consists of two atoms, one arising from NO3? and the other from different sources. This phenomenon is similar to codenitrification in fungi. The strain also evolved less, but significant, amounts of 15N15N from [15N]NO3? in addition to 15N15NO with concomitant cell growth. Prior to the production of N2 and N2O, NO3? was rapidly reduced to nitrite (NO2?) accompanied by distinct cell growth, showing that the actinomycete strain is a facultative anaerobe that depends on denitrification and nitrate respiration for anoxic growth. The cell-free activities of denitrifying enzymes could be reconstituted, supporting the notion that the 15N15N and 15N15NO species are produced by denitrification from NO3? via NO2?. We therefore demonstrated a unique system in an actinomycete that produces gaseous nitrogen (N2 and N2O) through both denitrification and codenitrification. The predominance of codenitrification over denitrification along with oxygen tolerance is the key feature of nitrate metabolism in this actinomycete.
机译：我们筛选了放线菌菌株产生二氮（N 2 ）的活性，并发现抗生素链霉菌 B-546会进化出N 2 和一氧化二氮（ N 2 O）来自硝酸盐（NO 3 ？）。从重同位素（[ 15 N] NO 3 ？）演化出来的N 2 大部分是< sup> 15 N 14 N，表明该氮原子由两个原子组成，一个原子由NO 3 ？和其他来自不同的来源。这种现象类似于真菌中的共硝化作用。该菌株还从[ 15 N] NO 3 进化出了数量较少但显着的 15 N 15 N除了 15 N 15 NO之外，还伴有？和细胞生长。在生产N 2 和N 2 O之前，NO 3 ？迅速还原为亚硝酸盐（NO 2 ？）伴随着明显的细胞生长，表明放线菌菌株是兼性厌氧菌，依赖于反硝化作用和硝酸盐呼吸来进行缺氧生长。可以重建反硝化酶的无细胞活性，从而支持 15 N 15 N和 15 N 15 < / sup>通过NO 2 ？通过NO 3 ？的反硝化作用产生NO物种。因此，我们证明了在放线菌中通过反硝化和共硝化产生气态氮（N 2 和N 2 O）的独特系统。共硝化作用优于反硝化作用以及耐氧性是该放线菌中硝酸盐代谢的关键特征。

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