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首页> 外文期刊>Journal of bacteriology >Role of the Transcriptional Regulator RamB (Rv0465c) in the Control of the Glyoxylate Cycle in Mycobacterium tuberculosis
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Role of the Transcriptional Regulator RamB (Rv0465c) in the Control of the Glyoxylate Cycle in Mycobacterium tuberculosis

机译：转录调节剂RamB（Rv0465c）在结核分枝杆菌中乙醛酸循环控制中的作用。

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Mycobacterium tuberculosis generally is assumed to depend on lipids as a major carbon and energy source when persisting within the host. The utilization of fatty acids requires a functional glyoxylate cycle with the key enzymes isocitrate lyase (Icl) and malate synthase. The open reading frame Rv0465c of M. tuberculosis H37Rv encodes a protein with significant sequence similarity to the transcriptional regulator RamB, which in Corynebacterium glutamicum controls the expression of several genes involved in acetate metabolism, i.e., those encoding enzymes of acetate activation and the glyoxylate cycle. We show here that the M. tuberculosis Rv0465c protein can functionally complement RamB in C. glutamicum and that it binds to the promoter regions of M. tuberculosis icl1 and Rv0465c. Construction and subsequent transcriptional and enzymatic analysis of a defined Rv0465c deletion mutant in M. tuberculosis revealed that the Rv0465c protein, now designated RamB, represses icl1 expression during growth with glucose and negatively autoregulates the expression of its own operon. Whole-genome microarray analysis of the M. tuberculosis ramB (ramBMT) mutant and the wild type furthermore showed that apart from icl1 and the ramBMT operon, the expression of all other M. tuberculosis genes involved in acetate metabolism remain unchanged in the mutant. Thus, RamBMT has a more specific regulatory function as RamB from C. glutamicum and is confined to expression control of icl1 and the ramBMT operon.
机译：一般认为，结核分枝杆菌在宿主体内持续存在时依赖脂质作为主要的碳和能源。脂肪酸的利用需要关键的酶异柠檬酸裂合酶（Icl）和苹果酸合酶进行乙醛酸循环。 M的开放阅读框Rv0465c。结核菌H37Rv编码一种与转录调节子RamB具有显着序列相似性的蛋白质，在谷氨酸棒杆菌中控制着参与乙酸盐代谢的几种基因的表达，即编码乙酸盐激活和代谢的酶。乙醛酸循环。我们在这里显示 M。结核病 Rv0465c蛋白在功能上可补充 C中的RamB。谷氨酸并与 M的启动子区域结合。结核病icl1 和Rv0465c。在 M中定义的Rv0465c缺失突变体的构建以及随后的转录和酶促分析。结核病表明，Rv0465c蛋白（现称为RamB）在葡萄糖生长过程中抑制 icl1 表达，并负调控自身操纵子的表达。全基因组微阵列分析。结核ramB （ ramB MT ）突变体和野生型进一步表明，除了 icl1 和 ramB MT 操纵子，所有其他 M的表达。突变体中与乙酸代谢有关的肺结核基因保持不变。因此，RamB MT 作为 C的RamB具有更具体的调节功能。谷氨酸，并仅限于 icl1 和 ramB MT 操纵子的表达控制。

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