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首页> 外文期刊>Journal of bacteriology >LuxS-Based Quorum Sensing Does Not Affect the Ability of Salmonella enterica Serovar Typhimurium To Express the SPI-1 Type 3 Secretion System, Induce Membrane Ruffles, or Invade Epithelial Cells
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LuxS-Based Quorum Sensing Does Not Affect the Ability of Salmonella enterica Serovar Typhimurium To Express the SPI-1 Type 3 Secretion System, Induce Membrane Ruffles, or Invade Epithelial Cells

机译：基于LuxS的群体感应不会影响沙门氏肠杆菌血清型鼠伤寒沙门氏菌表达SPI-1 3型分泌系统，诱导膜皱纹或侵袭上皮细胞的能力。

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摘要

Bacterial species can communicate by producing and sensing small autoinducer molecules by a process known as quorum sensing. Salmonella enterica produces autoinducer 2 (AI-2) via the luxS synthase gene, which is used by some bacterial pathogens to coordinate virulence gene expression with population density. We investigated whether the luxS gene might affect the ability of Salmonella enterica serovar Typhimurium to invade epithelial cells. No differences were found between the wild-type strain of S. Typhimurium, SL1344, and its isogenic luxS mutant with respect to the number and morphology of the membrane ruffles induced or their ability to invade epithelial cells. The dynamics of the ruffling process were also similar in the wild-type strain (SL1344) and the luxS mutant. Furthermore, comparing the Salmonella pathogenicity island 1 (SPI-1) type 3 secretion profiles of wild-type SL1344 and the luxS mutant by Western blotting and measuring the expression of a single-copy green fluorescent protein fusion to the prgH (an essential SPI-1 gene) promoter indicated that SPI-1 expression and activity are similar in the wild-type SL1344 and luxS mutant. Genetic deletion of luxS did not alter the virulence of S. Typhimurium in the mouse model, and therefore, it appears that luxS does not play a significant role in regulating invasion of Salmonella in vitro or in vivo.
机译：细菌物种可以通过称为群体感应的过程产生并感应小的自诱导分子来进行交流。 沙门氏菌通过 luxS 合酶基因产生自诱导子2（AI-2），某些细菌病原体使用该酶来调节毒力基因的表达与种群密度。我们研究了 luxS 基因是否会影响肠炎沙门氏菌血清鼠伤寒侵袭上皮细胞的能力。野生型 S 菌株之间没有发现差异。鼠伤寒沙门氏菌SL1344及其同基因 luxS 突变体在诱导的膜褶的数量和形态上或它们侵袭上皮细胞的能力方面。在野生型菌株（SL1344）和 luxS 突变体中，波纹的动力学过程也相似。此外，比较野生型SL1344和 luxS 突变体的沙门氏菌致产力 i sland 1（SPI-1）3型分泌谱通过蛋白质印迹和测量与 prgH （必需的SPI-1基因）启动子的单拷贝绿色荧光蛋白融合蛋白的表达，表明SPI-1在野生型中的表达和活性相似SL1344和 luxS 突变体。 luxS 的基因删除并没有改变 S 的毒力。小鼠模型中的鼠伤寒，因此， luxS 在体外或体内在调节沙门氏菌侵袭中没有发挥重要作用。

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