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首页> 外文期刊>Journal of bacteriology >Characterization of Alanine Catabolism in Pseudomonas aeruginosa and Its Importance for Proliferation In Vivo
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Characterization of Alanine Catabolism in Pseudomonas aeruginosa and Its Importance for Proliferation In Vivo

机译：铜绿假单胞菌中丙氨酸分解代谢的表征及其对体内增殖的重要性

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The opportunistic pathogen Pseudomonas aeruginosa causes a variety of infections in immunocompromised individuals, including individuals with the heritable disease cystic fibrosis. Like the carbon sources metabolized by many disease-causing bacteria, the carbon sources metabolized by P. aeruginosa at the host infection site are unknown. We recently reported that l-alanine is a preferred carbon source for P. aeruginosa and that two genes potentially involved in alanine catabolism (dadA and dadX) are induced during in vivo growth in the rat peritoneum and during in vitro growth in sputum (mucus) collected from the lungs of individuals with cystic fibrosis. The goals of this study were to characterize factors required for alanine catabolism in P. aeruginosa and to assess the importance of these factors for in vivo growth. Our results reveal that dadA and dadX are arranged in an operon and are required for catabolism of l-alanine. The dad operon is inducible by l-alanine, d-alanine, and l-valine, and induction is dependent on the transcriptional regulator Lrp. Finally, we show that a mutant unable to catabolize dl-alanine displays decreased competitiveness in a rat lung model of infection.
机译：机会性病原菌铜绿假单胞菌在免疫功能低下的个体中引起多种感染，包括患有遗传性疾病的囊性纤维化的个体。与许多致病细菌代谢的碳源一样， P代谢的碳源。宿主感染部位的铜绿不明。最近，我们报道了L-丙氨酸是 P的首选碳源。铜绿假单胞菌，以及在大鼠腹膜的体内生长过程和痰液的体外生长过程中诱导了两个潜在的与丙氨酸分解代谢有关的基因（ dadA 和 dadX ） （粘液）从患有囊性纤维化的个体的肺中收集。这项研究的目的是表征 P中丙氨酸分解代谢所需的因子。铜绿假单胞菌并评估这些因素对体内生长的重要性。我们的结果表明， dadA 和 dadX 排列在操纵子中，是l-丙氨酸分解代谢所必需的。 dad 操纵子可被l-丙氨酸，d-丙氨酸和l-缬氨酸诱导，并且诱导依赖于转录调节因子Lrp。最后，我们显示出一个无法分解代谢dl-丙氨酸的突变体在大鼠肺部感染模型中显示出降低的竞争能力。

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