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首页> 外文期刊>Journal of bacteriology >Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor
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Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor

机译：霍乱弧菌TcpF定殖因子分泌和功能决定簇的遗传图谱。

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Colonization of the human small intestine by Vibrio cholerae requires the type IV toxin-coregulated pilus (TCP). TcpF, which is encoded within the tcp operon, is secreted from the bacterial cell by the TCP apparatus and is also essential for colonization. Bacteria lacking tcpF are deficient in colonization, and anti-TcpF antibodies are protective in the infant mouse cholera model. In order to elucidate the regions of the protein that are required for secretion through the TCP apparatus and for its function in colonization, random mutagenesis of tcpF was performed. Analysis of these mutants suggests that multiple regions throughout the protein influence extracellular secretion and that determinants near the C terminus are important for the function of TcpF in colonization. The TcpF proteins of certain environmental V. cholerae isolates with 31% to 66% identity to pathogenic V. cholerae TcpF showed higher similarity in regions identified as secretion determinants but diverged in regions found to be important for colonization. These environmental TcpF proteins are secreted from the pathogenic strain; however, they do not mediate colonization in the infant mouse model. Here we provide genetic evidence pointing toward regions of TcpF that influence secretion, as well as regions that play an important role in in vivo colonization.
机译：霍乱弧菌定植在人的小肠需要IV型毒素结合菌毛（TCP）。 TcpF编码在 tcp 操纵子中，由TCP装置从细菌细胞中分泌出来，并且对于定植也是必不可少的。缺乏 tcpF 的细菌在定植中缺乏，而抗TcpF抗体在婴儿霍乱模型中具有保护作用。为了阐明通过TCP设备分泌所需的蛋白质区域及其在定居中的功能，对 tcpF 进行了随机诱变。对这些突变体的分析表明，整个蛋白质中的多个区域会影响细胞外分泌，C末端附近的决定簇对于TcpF在定植中的功能很重要。某些环境V的TcpF蛋白。霍乱与病原体 V具有31％至66％的同一性。霍乱弧菌TcpF在被确定为分泌决定因素的区域显示出较高的相似性，但在被认为对定植重要的区域之间却存在分歧。这些环境中的TcpF蛋白是由致病菌株分泌的。但是，它们不介导婴儿小鼠模型中的定植。在这里，我们提供了遗传证据，这些证据指向影响分泌的TcpF区域以及在体内定殖中起重要作用的区域。

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