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首页> 外文期刊>Journal of bacteriology >Brochothrix thermosphacta Bacteriophages Feature Heterogeneous and Highly Mosaic Genomes and Utilize Unique Prophage Insertion Sites
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Brochothrix thermosphacta Bacteriophages Feature Heterogeneous and Highly Mosaic Genomes and Utilize Unique Prophage Insertion Sites

机译：Brochothrix thermosphacta噬菌体具有异质和高度镶嵌的基因组，并利用独特的噬菌体插入位点

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Brochothrix belongs to the low-GC branch of Gram-positive bacteria (Firmicutes), closely related to Listeria, Staphylococcus, Clostridium, and Bacillus. Brochothrix thermosphacta is a nonproteolytic food spoilage organism, adapted to growth in vacuum-packaged meats. We report the first genome sequences and characterization of Brochothrix bacteriophages. Phage A9 is a myovirus with an 89-nm capsid diameter and a 171-nm contractile tail; it belongs to the Spounavirinae subfamily and shares significant homologies with Listeria phage A511, Staphylococcus phage Twort, and others. The A9 unit genome is 127 kb long with 11-kb terminal redundancy; it encodes 198 proteins and 6 tRNAs. Phages BL3 and NF5 are temperate siphoviruses with a head diameter of 56 to 59 nm. The BL3 tail is 270 nm long, whereas NF5 features a short tail of only 94 nm. The NF5 genome (36.95 kb) encodes 57 gene products, BL3 (41.52 kb) encodes 65 products, and both are arranged in life cycle-specific modules. Surprisingly, BL3 and NF5 show little relatedness to Listeria phages but rather demonstrate relatedness to lactococcal phages. Peptide mass fingerprinting of viral proteins indicate programmed ?1 translational frameshifts in the NF5 capsid and the BL3 major tail protein. Both NF5 and BL3 feature circularly permuted, terminally redundant genomes, packaged by a headful mechanism, and integrases of the serine (BL3) and tyrosine (NF5) types. They utilize unique target sequences not previously described: BL3 inserts into the 3′ end of a RNA methyltransferase, whereas NF5 integrates into the 5′-terminal part of a putative histidinol-phosphatase. Interestingly, both genes are reconstituted by phage sequence.
机译： Brochothrix 属于革兰氏阳性菌（ Firmicutes ）的低GC分支，与李斯特菌，葡萄球菌，梭状芽孢杆菌和芽孢杆菌。 Brochothrix thermosphacta 是一种非蛋白水解性食物腐败生物，适合在真空包装的肉中生长。我们报告了 Brochothrix 噬菌体的第一个基因组序列和特征。噬菌体A9是一种肌病毒，衣壳直径为89纳米，尾巴收缩为171纳米。它属于 Spounavirinae 子家族，与 Listeria 噬菌体A511，葡萄球菌噬菌体Twort具有重要的同源性。 A9单位基因组长127 kb，末端冗余11 kb;它编码198种蛋白质和6种tRNA。噬菌体BL3和NF5是温和的头孢病毒，其头部直径为56至59nm。 BL3尾巴长270 nm，而NF5尾巴短只有94 nm。 NF5基因组（36.95 kb）编码57个基因产物，BL3（41.52 kb）编码65个产物，两者都排列在生命周期特定的模块中。令人惊讶地，BL3和NF5与李斯特菌噬菌体几乎没有相关性，而是与乳球菌噬菌体相关。病毒蛋白的肽质量指纹图谱表明NF5衣壳和BL3主要尾巴蛋白中有程序化的？1翻译移码。 NF5和BL3均具有环状排列，末端冗余的基因组，并通过有力的机制进行包装，并整合了丝氨酸（BL3）和酪氨酸（NF5）类型。它们利用先前未描述的独特靶序列：BL3插入RNA甲基转移酶的3'末端，而NF5整合到假定的组蛋白醇磷酸酶的5'末端部分。有趣的是，两个基因均由噬菌体序列重构。

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