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首页> 外文期刊>Journal of bacteriology >FadD Is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids
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FadD Is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids

机译：FadD是利用膜脂释放的内源性脂肪酸所必需的

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FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation of fadD in the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti promotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found that S. meliloti fadD mutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids. Escherichia coli fadD mutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant of E. coli with a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids in fadD mutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed with S. meliloti revealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth.
机译：FadD是一种酰基辅酶A（CoA）合成酶，负责将外源长链脂肪酸（LCFA）活化为酰基CoA。共生固氮菌中苜蓿中华根瘤菌中 fadD 的突变会促进群体运动，并导致苜蓿植物结瘤的缺陷。在这项研究中，我们发现S. meliloti fadD 突变体在生长的稳定期积累了游离脂肪酸的混合物。游离脂肪酸池的组成和在用Δ5-去饱和酶（Δ5-Des）特异性标记酯化脂肪酸后获得的结果与膜磷脂是释放的脂肪酸的来源一致。大肠杆菌的 fadD 突变体还在固定相中积累了从膜脂释放的游离脂肪酸。这种现象在FadL脂肪酸转运蛋白不足的大肠杆菌突变体中没有发生，这表明 fadD 突变体中脂肪酸的积累发生在细胞内部。我们的结果表明，除了激活外源性LCFA外，细菌FadD在激活从膜脂释放的内源性脂肪酸中也起着重要作用。此外，用苜蓿链球菌进行的表达分析表明，功能性FadD是上调与脂肪酸降解有关的基因所必需的，并表明在野生型菌株中，膜脂释放的脂肪酸通过β-氧化降解。生长的稳定阶段。

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