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首页> 外文期刊>Journal of bacteriology >The syp Enhancer Sequence Plays a Key Role in Transcriptional Activation by the σ54-Dependent Response Regulator SypG and in Biofilm Formation and Host Colonization by Vibrio fischeri
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The syp Enhancer Sequence Plays a Key Role in Transcriptional Activation by the σ54-Dependent Response Regulator SypG and in Biofilm Formation and Host Colonization by Vibrio fischeri

机译：syp增强子序列在由σ54依赖性应答调节剂SypG激活的转录激活中以及在费氏弧菌的生物膜形成和宿主定殖中起关键作用

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Biofilm formation by Vibrio fischeri is a complex process that requires multiple regulators. One such regulator, the NtrC-like response regulator SypG, controls biofilm formation and host colonization by V. fischeri via its impact on transcription of the symbiosis polysaccharide (syp) locus. SypG is predicted to activate syp transcription by binding to the syp enhancer (SE), a conserved sequence located upstream of four syp promoters. In this study, we performed an in-depth analysis of the sequences necessary for SypG to promote syp transcription and biofilm formation. We found that the SE sequence is necessary for SypG-mediated syp transcription, identified individual bases necessary for efficient activation, and determined that SypG is able to bind to syp promoter regions. We also identified SE sequences outside the syp locus and established that SypG recognizes these sequences as well. Finally, deletion of the SE sequence upstream of sypA led to defects in both biofilm formation and host colonization that could be restored by reintroducing the SE sequence into its native location in the chromosome. This work thus fills in critical gaps in knowledge of the Syp regulatory circuit by demonstrating a role for the SE sequence in SypG-dependent control of biofilm formation and host colonization and by identifying new putative regulon members. It may also provide useful insights into other bacteria, such as Vibrio vulnificus and Vibrio parahaemolyticus, that have syp-like loci and conserved SE sequences.
机译：费氏弧菌形成生物膜的过程很复杂，需要多个调节器。一种这样的调节剂，即类似NtrC的应答调节剂SypG，通过其对共生多糖（ syp ）基因座转录的影响来控制费氏弧菌的生物膜形成和宿主定植。预计SypG通过与 syp 增强子（SE）结合来激活 syp 转录，SE是位于四个 syp 启动子上游的保守序列。在这项研究中，我们对SypG促进 syp 转录和生物膜形成所必需的序列进行了深入分析。我们发现SE序列是SypG介导的 syp 转录所必需的，确定了有效激活所必需的各个碱基，并确定SypG能够与 syp 启动子区域结合。我们还确定了 syp 位点之外的SE序列，并确定SypG也可以识别这些序列。最后，删除 sypA 上游的SE序列会导致生物膜形成和宿主定植的缺陷，可以通过将SE序列重新引入染色体的天然位置来恢复。因此，这项工作通过证明SE序列在依赖SypG的生物膜形成和寄主定植控制中的作用以及确定新的推定的调节子成员，填补了Syp调控电路知识的关键空白。它也可能提供对其他细菌的有用的见解，例如创伤弧菌和副溶血弧菌，它们具有 syp 样位点和保守的SE序列。

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