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首页> 外文期刊>Journal of bacteriology >Outer Membrane Proteins of Fibrobacter succinogenes with Potential Roles in Adhesion to Cellulose and in Cellulose Digestion
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Outer Membrane Proteins of Fibrobacter succinogenes with Potential Roles in Adhesion to Cellulose and in Cellulose Digestion

机译：琥珀酸纤维杆菌外膜蛋白在纤维素粘附和纤维素消化中具有潜在作用

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Comparative analysis of binding of intact glucose-grown Fibrobacter succinogenes strain S85 cells and adhesion-defective mutants AD1 and AD4 to crystalline and acid-swollen (amorphous) cellulose showed that strain S85 bound efficiently to both forms of cellulose while mutant Ad1 bound to acid-swollen cellulose, but not to crystalline cellulose, and mutant Ad4 did not bind to either. One- and two-dimensional electrophoresis (2-DE) of outer membrane cellulose binding proteins and of outer membranes, respectively, of strain S85 and adhesion-defective mutant strains in conjunction with mass spectrometry analysis of tryptic peptides was used to identify proteins with roles in adhesion to and digestion of cellulose. Examination of the binding to cellulose of detergent-solubilized outer membrane proteins from S85 and mutant strains revealed six proteins in S85 that bound to crystalline cellulose that were absent from the mutants and five proteins in Ad1 that bound to acid-swollen cellulose that were absent from Ad4. Twenty-five proteins from the outer membrane fraction of cellulose-grown F. succinogenes were identified by 2-DE, and 16 of these were up-regulated by growth on cellulose compared to results with growth on glucose. A protein identified as a Cl-stimulated cellobiosidase was repressed in S85 cells growing on glucose and further repressed in the mutants, while a cellulose-binding protein identified as pilin was unchanged in S85 grown on glucose but was not produced by the mutants. The candidate differential cellulose binding proteins of S85 and the mutants and the proteins induced by growth of S85 on cellulose provide the basis for dissecting essential components of the cellulase system of F. succinogenes.
机译：完整葡萄糖生长的琥珀酸纤维杆菌菌株S85细胞和粘附缺陷型突变体AD1和AD4与晶体和酸膨胀（无定形）纤维素结合的比较分析表明，菌株S85有效结合两种形式的纤维素而突变体Ad1与酸溶胀的纤维素结合，但与结晶纤维素不结合，而突变体Ad4与两者均不结合。 S85菌株和粘附缺陷型突变菌株的外膜纤维素结合蛋白和外膜的一维和二维电泳（2-DE）结合胰蛋白酶肽的质谱分析用于鉴定具有作用的蛋白对纤维素的粘附和消化。检查来自S85和突变菌株的去污剂增溶的外膜蛋白与纤维素的结合，发现S85中有6个蛋白与突变体不存在的结晶纤维素结合，而Ad1中有5个蛋白与不存在于酸溶胀的纤维素中结合的蛋白质。广告4。来自纤维素生长的 F的外膜部分的二十五个蛋白质。通过2-DE鉴定出琥珀酸基因，其中的16种通过在纤维素上的生长而上调，而在葡萄糖上的生长则被上调。被鉴定为Cl刺激的纤维二糖苷酶的蛋白质在葡萄糖上生长的S85细胞中被阻遏，并且在突变体中被进一步抑制，而被鉴定为菌毛蛋白的纤维素结合蛋白在葡萄糖上生长的S85中没有变化，但不是由突变体产生。 S85和突变体的候选差异纤维素结合蛋白以及由S85在纤维素上生长诱导的蛋白为解剖 F的纤维素酶系统的必需成分提供了基础。琥珀酸。

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