Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element

Laetitia Abdou; Céline Boileau; Pascale de Philip; Sandrine Pagès; Henri-Pierre Fiérobe; Chantal Tardif

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Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element

机译：溶纤梭菌cip-cel操纵子的转录调控：涉及分解代谢物响应元素的复杂机制。

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The cip-cel cluster of genes plays an important role in the catabolism of the substrate cellulose by Clostridium cellulolyticum. It encodes several key components of the cellulosomes, including the scaffolding protein CipC and the major cellulase Cel48F. All the genes of this cluster display linked transcription, focusing attention on the promoter upstream from the first gene, cipC. We analyzed the regulation of the cipC promoter using a transcriptional fusion approach. A single promoter is located between nucleotides ?671 and ?643 with respect to the ATG start codon, and the large mRNA leader sequence is processed at position ?194. A catabolite-responsive element (CRE) 414 nucleotides downstream from the transcriptional start site has been shown to be involved in regulating this operon by a carbon catabolite repression mechanism. This CRE is thought to bind a CcpA-like regulator complexed with a P-Ser-Crh-like protein. Sequences surrounding the promoter sequence may also be involved in direct (sequence-dependent DNA curvature) or indirect (unknown regulator binding) regulation.

机译： cip-cel 基因簇在解纤梭菌对底物纤维素的分解代谢中起重要作用。它编码纤维素酶体的几个关键成分，包括支架蛋白CipC和主要的纤维素酶Cel48F。该簇的所有基因都显示连锁转录，将注意力集中在第一个基因 cipC 上游的启动子上。我们使用转录融合方法分析了 cipC 启动子的调控。相对于ATG起始密码子，单个启动子位于核苷酸？671和？643之间，并且大的mRNA前导序列在位置？194被加工。转录起始位点下游的分解代谢物响应元件（CRE）414个核苷酸已显示参与通过碳分解物抑制机制调节该操纵子。该CRE被认为结合了与P-Ser-Crh样蛋白复合的CcpA样调节剂。启动子序列周围的序列也可能参与直接（序列依赖性DNA弯曲）或间接（未知调节子结合）调控。 展开▼

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《Journal of bacteriology》 |2008年第5期|共8页

作者
Laetitia Abdou; Céline Boileau; Pascale de Philip; Sandrine Pagès; Henri-Pierre Fiérobe; Chantal Tardif;
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1. Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element [J] . Laetitia Abdou, Céline Boileau, Pascale de Philip, Journal of bacteriology . 2008,第5期

机译：溶纤梭菌cip-cel操纵子的转录调控：涉及分解代谢物响应元素的复杂机制。

2. Regulation of the CDP-choline pathway by sterol regulatory element binding proteins involves transcriptional and post-transcriptional mechanisms [J] . Ridgway ND., Lagace TA. The Biochemical Journal . 2003,第Pta3期

机译：固醇调节元件结合蛋白对CDP-胆碱途径的调节涉及转录和转录后机制

3. Regulation of the CDP-choline pathway by sterol regulatory element binding proteins involves transcriptional and post-transcriptional mechanisms [J] . Ridgway ND., Lagace TA. The Biochemical Journal . 2003,第Pta3期

机译：固醇调节元件结合蛋白对CDP-胆碱途径的调节涉及转录和转录后机制

4. Structure of the Eukaryotic Transcription Machinery: Insights into theMechanism of Transcription Initiation and Regulation [C] . Francisco J. Asturias, Roger D. Kornberg, John L. Craighead, Microscopy Society of America annual meeting;Microscopy Society of Canada annual meeting;Societe de Microscopie du Canada annual meeting;Microbeam Analysis Society annual meeting;International Metallographic Society annual meeting . 2002

机译：真核转录机制的结构：转录启动和调控机制的见解。

5. Investigating Additional Roles of the trp RNA-Binding Attenuation Protein (Trap) in Transcriptional Regulation of the trp Operon in Bacillus subtilis [D] . Szyjka, Courtney Elizabeth. 2021

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6. Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element [O] . Laetitia Abdou, Céline Boileau, Pascale de Philip, 2008

机译：溶纤梭菌cip-cel操纵子的转录调控：涉及分解代谢物响应元素的复杂机制。

7. Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element▿ [O] . Abdou, Laetitia, Boileau, Céline, de Philip, Pascale, 2007

机译：溶纤梭菌cip-cel操纵子的转录调控：涉及分解代谢物响应元素的复杂机制▿

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Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element

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