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首页> 外文期刊>Journal of bacteriology >Comparative Genome Biology of a Serogroup B Carriage and Disease Strain Supports a Polygenic Nature of Meningococcal Virulence
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Comparative Genome Biology of a Serogroup B Carriage and Disease Strain Supports a Polygenic Nature of Meningococcal Virulence

机译：B血清型携带和疾病菌株的比较基因组生物学支持脑膜炎球菌毒力的多基因性质。

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Neisseria meningitidis serogroup B strains are responsible for most meningococcal cases in the industrialized countries, and strains belonging to the clonal complex ST-41/44 are among the most prevalent serogroup B strains in carriage and disease. Here, we report the first genome and transcriptome comparison of a serogroup B carriage strain from the clonal complex ST-41/44 to the serogroup B disease strain MC58 from the clonal complex ST-32. Both genomes are highly colinear, with only three major genome rearrangements that are associated with the integration of mobile genetic elements. They further differ in about 10% of their gene content, with the highest variability in gene presence as well as gene sequence found for proteins involved in host cell interactions, including Opc, NadA, TonB-dependent receptors, RTX toxin, and two-partner secretion system proteins. Whereas housekeeping genes coding for metabolic functions were highly conserved, there were considerable differences in their expression pattern upon adhesion to human nasopharyngeal cells between both strains, including differences in energy metabolism and stress response. In line with these genomic and transcriptomic differences, both strains also showed marked differences in their in vitro infectivity and in serum resistance. Taken together, these data support the concept of a polygenic nature of meningococcal virulence comprising differences in the repertoire of adhesins as well as in the regulation of metabolic genes and suggest a prominent role for immune selection and genetic drift in shaping the meningococcal genome.
机译：在工业化国家，脑膜炎奈瑟菌血清群B株是大多数脑膜炎球菌病例的病因，属于克隆复合体ST-41 / 44的菌株在运输和疾病中是最流行的B血清群株。在这里，我们报告了来自克隆复合体ST-41 / 44的血清群B携带株与来自克隆复合体ST-32的血清群B疾病株MC58的首次基因组和转录组比较。两个基因组都是高度共线的，只有三个主要的基因组重排与移动遗传元件的整合有关。它们的基因含量进一步差异约10％，在基因存在以及涉及宿主细胞相互作用的蛋白质（包括Opc，NadA，TonB依赖性受体，RTX毒素和两分子伴侣）中发现的基因序列的变异性最高。分泌系统蛋白。尽管编码代谢功能的管家基因是高度保守的，但是两种菌株在粘附于人鼻咽细胞后其表达方式存在相当大的差异，包括能量代谢和应激反应的差异。符合这些基因组和转录组学差异，这两个菌株在体外感染性和血清抗性方面也显示出明显差异。综上所述，这些数据支持了脑膜炎球菌毒力的多基因性质的概念，包括粘附素库以及代谢基因调控方面的差异，并暗示了免疫选择和遗传漂移在塑造脑膜炎球菌基因组中的重要作用。

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