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首页> 外文期刊>Journal of bacteriology >Two DNA Translocases Synergistically Affect Chromosome Dimer Resolution in Bacillus subtilis
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Two DNA Translocases Synergistically Affect Chromosome Dimer Resolution in Bacillus subtilis

机译:两个DNA Translocases协同影响枯草芽孢杆菌的染色体二聚体分辨率。

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摘要

In Bacillus subtilis, chromosome dimers that block complete segregation of sister chromosomes arise in about 15% of exponentially growing cells. Two dedicated recombinases, RipX and CodV, catalyze the resolution of dimers by site-specific recombination at the dif site, which is located close to the terminus region on the chromosome. We show that the two DNA translocases in B. subtilis, SftA and SpoIIIE, synergistically affect dimer resolution, presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively. Furthermore, we observed that both recombinases, RipX and CodV, assemble on the chromosome at the dif site throughout the cell cycle. The preassembly of recombinases probably ensures that dimer resolution can occur rapidly within a short time window around cell division.
机译:在<枯草芽孢杆菌中,阻止姐妹染色体完全分离的染色体二聚体出现在约15%的指数生长细胞中。两种专用的重组酶RipX和CodV通过位于 dif 位点(靠近染色体末端区域)的位点特异性重组来催化二聚体的分离。我们显示了 B中的两个DNA转位酶。枯草,SftA和SpoIIIE协同影响二聚体的分辨率,大概是通过在细胞分裂完成之前或之后将 dif 位点紧靠放置。此外,我们观察到在整个细胞周期中,重组酶RipX和CodV都在染色体上的 dif 位点组装。重组酶的预装配可能确保二聚体分辨率可以在细胞分裂的短时间内迅速发生。

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