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首页> 外文期刊>Journal of bacteriology >Rational Design of an Artificial Genetic Switch: Co-Option of the H-NS-Repressed proU Operon by the VirB Virulence Master Regulator
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Rational Design of an Artificial Genetic Switch: Co-Option of the H-NS-Repressed proU Operon by the VirB Virulence Master Regulator

机译：人工遗传开关的合理设计：由VirB毒力主调节器共同抑制H-NS抑制的proU操纵子

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The H-NS protein represses the transcription of hundreds of genes in Gram-negative bacteria. Derepression is achieved by a multitude of mechanisms, many of which involve the binding of a protein to DNA at the repressed promoter in a manner that compromises the maintenance of the H-NS–DNA nucleoprotein repression complex. The principal virulence gene promoters in Shigella flexneri, the cause of bacillary dysentery, are repressed by H-NS. VirB, a protein that closely resembles members of the ParB family of plasmid-partitioning proteins, derepresses the operons that encode the main structural components and the effector proteins of the S. flexneri type III secretion system. Bioinformatic analysis suggests that VirB has been co-opted into its current role as an H-NS antagonist in S. flexneri. To test this hypothesis, the potential for VirB to act as a positive regulator of proU, an operon that is repressed by H-NS, was assessed. Although VirB has no known relationship with the osmoregulated proU operon, it could relieve H-NS-mediated repression when the parS-like VirB binding site was placed appropriately upstream of the RpoD-dependent proU promoter. These results reveal the remarkable facility with which novel regulatory circuits can evolve, at least among those promoters that are repressed by H-NS.
机译：H-NS蛋白可抑制革兰氏阴性细菌中数百个基因的转录。抑制是通过多种机制实现的，其中许多机制涉及蛋白质与被抑制启动子处的DNA结合，从而损害了H-NS-DNA核蛋白抑制复合物的维持。 H-NS抑制了痢疾志贺菌中的主要毒力基因启动子，这是细菌性痢疾的病因。 VirB是一种非常类似于ParB家族的质粒分配蛋白成员的蛋白，可抑制编码弗氏链球菌III型分泌系统主要结构成分和效应蛋白的操纵子。生物信息学分析表明，VirB已被选作目前在弗氏链球菌中作为H-NS拮抗剂的角色。为了验证这一假设，评估了VirB可能作为 proU （一种被H-NS抑制的操纵子）的阳性调节子的潜力。尽管VirB与渗透的 proU 操纵子没有已知的关系，但当 parS 样VirB结合位点适当地位于RpoD的上游时，它可以缓解H-NS介导的阻遏依赖的 proU 启动子。这些结果表明，至少在被H-NS抑制的那些启动子中，新颖的调节电路可以进化的显着功能。

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