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首页> 外文期刊>Journal of bacteriology >The Thiamine Kinase (YcfN) Enzyme Plays a Minor but Significant Role in Cobinamide Salvaging in Salmonella enterica
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The Thiamine Kinase (YcfN) Enzyme Plays a Minor but Significant Role in Cobinamide Salvaging in Salmonella enterica

机译：硫胺素激酶（YcfN）酶在肠道沙门氏菌中的Cobinamide挽救中起着次要但重要的作用

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摘要

Cobinamide (Cbi) salvaging is impaired, but not abolished, in a Salmonella enterica strain lacking a functional cobU gene. CobU is a bifunctional enzyme (NTP:adenosylcobinamide [NTP:AdoCbi] kinase, GTP:adenosylcobinamide-phosphate [GTP:AdoCbi-P] guanylyltransferase) whose AdoCbi kinase activity is necessary for Cbi salvaging in this bacterium. Inactivation of the ycfN gene in a ΔcobU strain abrogated Cbi salvaging. Introduction of a plasmid carrying the ycfN+ allele into a ΔcobU ΔycfN strain substantially restored Cbi salvaging. Mass spectrometry data indicate that when YcfN-enriched cell extracts were incubated with AdoCbi and ATP, the product of the reaction was AdoCbi-P. Results from bioassays confirmed that YcfN converted AdoCbi to AdoCbi-P in an ATP-dependent manner. YcfN is a good example of enzymes that are used by the cell in multiple pathways to ensure the salvaging of valuable precursors.
机译：在缺乏功能性 cobU 基因的 Salmonella enterica 菌株中，Cobinamide（Cbi）挽救功能受到损害，但并未消除。 CobU是一种双功能酶（NTP：腺苷酸合氨酰胺[NTP：AdoCbi]激酶，GTP：腺苷酸钴合酰胺-磷酸[GTP：AdoCbi-P]胍基转移酶），其AdoCbi激酶活性对于在该细菌中挽救Cbi是必需的。 Δ cobU 菌株中 ycfN 基因的失活消除了Cbi的挽救。将携带 ycfN + 等位基因的质粒引入Δ cobU Δ ycfN 菌株中，基本上恢复了Cbi挽救。质谱数据表明，将富含YcfN的细胞提取物与AdoCbi和ATP孵育时，反应产物为AdoCbi-P。生物测定的结果证实，YcfN以ATP依赖的方式将AdoCbi转化为AdoCbi-P。 YcfN是细胞在多种途径中用来确保有价值的前体得到挽救的酶的一个很好的例子。

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