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首页> 外文期刊>Journal of bacteriology >An Extremely Oligotrophic Bacterium, Rhodococcus erythropolis N9T-4, Isolated from Crude Oil
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An Extremely Oligotrophic Bacterium, Rhodococcus erythropolis N9T-4, Isolated from Crude Oil

机译：从原油中分离出来的极少营养细菌，红球菌N9T-4

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Rhodococcus erythropolis N9T-4, which was isolated from crude oil, showed extremely oligotrophic growth and formed its colonies on a minimal salt medium solidified using agar or silica gel without any additional carbon source. N9T-4 did not grow under CO2-limiting conditions but could grow on a medium containing NaHCO3 under the same conditions, suggesting that the oligotrophic growth of N9T-4 depends on CO2. Proteomic analysis of N9T-4 revealed that two proteins, with molecular masses of 45 and 55 kDa, were highly induced under the oligotrophic conditions. The primary structures of these proteins exhibited striking similarities to those of methanol: N,N′-dimethyl-4-nitrosoaniline oxidoreductase and an aldehyde dehydrogenase from Rhodococcus sp. These enzyme activities were three times higher under oligotrophic conditions than under n-tetradecane-containing heterotrophic conditions, and gene disruption for the aldehyde dehydrogenase caused a lack of growth on the minimal salt medium. Furthermore, 3-hexulose 6-phosphate synthase and phospho-3-hexuloisomerase activities, which are key enzymes in the ribulose monophosphate pathway in methylotrophic bacteria, were detected specifically in the cell extract of oligotrophically grown N9T-4. These results suggest that CO2 fixation involves methanol (formaldehyde) metabolism in the oligotrophic growth of R. erythropolis N9T-4.
机译：从原油中分离出的 Rhodococcus erythropolis N9T-4表现出极富营养性，并在琼脂或硅胶固化的最小盐培养基上形成菌落，没有任何其他碳源。 N9T-4不能在CO 2 限制条件下生长，但可以在相同条件下在含有NaHCO 3 的培养基上生长，这表明N9T-4的贫营养生长取决于在CO 2 上。 N9T-4的蛋白质组学分析表明，在贫营养条件下，两种蛋白质的分子量分别为45和55 kDa。这些蛋白质的一级结构与甲醇具有显着相似性： N ， N '-二甲基-4-亚硝基苯胺氧化还原酶和来自红球菌的醛脱氢酶。 em> sp。这些酶的活性在低营养条件下比在含 n -十四烷的异营养条件下高三倍，并且醛脱氢酶的基因破坏导致在最小盐培养基上缺乏生长。此外，在寡营养生长的N9T-4的细胞提取物中，具体检测到甲基营养菌核糖单磷酸途径中的关键酶3-己糖6-磷酸合酶和磷酸-3-己糖异构酶活性。这些结果表明，CO 2 的固定涉及 R贫营养生长中的甲醇（甲醛）代谢。 erythropolis N9T-4。

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