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首页> 外文期刊>Journal of bacteriology >Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose by Sulfolobus acidocaldarius
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Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose by Sulfolobus acidocaldarius

机译：Sulfolobus acidocaldarius同时利用葡萄糖和木糖的过程中不存在Diauxie

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Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilization. The organism utilized a mixture of 1 g/liter of each sugar simultaneously with a specific growth rate of 0.079 h?1 and showed no preference for the order in which it utilized each sugar. The organism grew faster on 2 g/liter xylose (0.074 h?1) as the sole carbon source than on an equal amount of glucose (0.022 h?1). When grown on a mixture of the two carbon sources, the growth rate of the organism increased from 0.052 h?1 to 0.085 h?1 as the ratio of xylose to glucose increased from 0.25 to 4. S. acidocaldarius appeared to utilize a mixture of glucose and xylose at a rate roughly proportional to their concentrations in the medium, resulting in complete utilization of both sugars at about the same time. Gene expression in cells grown on xylose alone was very similar to that in cells grown on a mixture of xylose and glucose and substantially different from that in cells grown on glucose alone. The mechanism by which the organism utilized a mixture of sugars has yet to be elucidated.
机译： Sulfolobus acidocaldarius 利用葡萄糖和木糖作为唯一碳源，但其同时代谢这些糖的能力尚不清楚。我们报道在 S的生长过程中不存在diauxie。葡萄糖和木糖为辅碳源的酸性嗜酸菌。葡萄糖的存在不抑制木糖利用率。该生物体同时使用每克糖1克/升的混合物，比生长速率为0.079 h ？1 ，对每种糖的使用顺序没有偏爱。生物体在2 g /升木糖（0.074 h ？1 ）作为唯一碳源时的生长速度快于等量的葡萄糖（0.022 h ？1 ）。当在两种碳源的混合物上生长时，随着木糖与葡萄糖的比例增加，生物体的生长速率从0.052 h ？1 增加到0.085 h ？1 从0.25到4。 acidocaldarius 似乎以与葡萄糖和木糖在培养基中的浓度大致成正比的比率利用了葡萄糖和木糖的混合物，从而导致两种糖在大约同一时间完全被利用。单独在木糖上生长的细胞中的基因表达与在木糖和葡萄糖的混合物上生长的细胞中的基因表达非常相似，并且与单独在葡萄糖上生长的细胞中的基因表达非常不同。有机体利用糖的混合物的机制尚未阐明。

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