Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis

Shiyong Chen; Ozan S. Kumru; Wolfram R. Zückert

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Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis

机译：表面蛋白水解法测定疏螺旋体表面脂蛋白锚固拓扑

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We used a surface trypsinolysis assay to probe accessibility of the membrane-proximal N-terminal tether peptides of Borrelia surface lipoproteins OspA and Vsp1. Our findings with both wild-type and mutant proteins are only compatible with the anchoring of these surface lipoproteins in the outer leaflet of the outer spirochetal membrane.

机译：我们使用了一种表面胰蛋白酶解试验来探查伯氏疏螺旋体表面脂蛋白OspA和Vsp1的膜近端N末端系链肽的可及性。我们对野生型和突变型蛋白的发现仅与这些表面脂蛋白在螺旋体外膜的小叶中的锚定相容。

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《Journal of bacteriology》 |2011年第22期|共5页
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Shiyong Chen; Ozan S. Kumru; Wolfram R. Zückert;
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中图分类微生物学;
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1. Tissue Inhibitor of Metalloproteinase-1 Promotes Polymorphonuclear Neutrophil (PMN) Pericellular Proteolysis by Anchoring Matrix Metalloproteinase-8 and-9 to PMN Surfaces [J] . Wang Xiaoyun, Rojas-Quintero Joselyn, Wilder Julie, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2019,第11期

机译：金属蛋白酶-1的组织抑制剂通过将基质金属蛋白酶-8和-9和PMN表面锚定，促进多晶核性粒细胞（PMN）围粒体溶液
2. Limited surface exposure of Borrelia burgdorferi outer surface lipoproteins [J] . David L. Cox, Darrin R. Akins, Ken W. Bourell Proceedings of the National Academy of Sciences of the United States of America . 1996,第15期

机译：伯氏疏螺旋体外表面脂蛋白的有限表面暴露
3. Site-Specific Proteolysis of the MALP-404 Lipoprotein Determines the Release of a Soluble Selective Lipoprotein-Associated Motif-Containing Fragment and Alteration of the Surface Phenotype of Mycoplasma fermentans [J] . Kelley L. Davis, Kim S. Wise Infection and immunity . 2002,第3期

机译：MALP-404脂蛋白的特定于位点的蛋白水解确定可溶性选择性脂蛋白相关的母体片段的释放和发酵支原体表面表型的改变
4. Experimental Determination of Polar Anchoring Strength for a Hybrid Aligned Nematic Liquid Crystal Cell with a Boundary Continuously Varying in Its Surface Energy [C] . Andrew D. Price a, Daniel K. Schwartz International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference . 2005

机译：杂交对齐向液晶单元极锚固强度的实验测定与其表面能连续变化的边界连续变化
5. Role of surface topology on wetting of complex surfaces. [D] . Sanchez Santiago, Javier. 2013

机译：表面拓扑在复杂表面润湿中的作用。
6. Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis [O] . Shiyong Chen, Ozan S. Kumru, Wolfram R. Zückert 2011

机译：表面蛋白水解法测定疏螺旋体表面脂蛋白锚的拓扑结构
7. Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis ▿ [O] . Chen, Shiyong, Kumru, Ozan S., Zückert, Wolfram R. 2011

机译：表面蛋白水解法测定疏螺旋体表面脂蛋白锚的拓扑结构 ▿
8. Theoretical Model Applicable to the Experimental Determination of Surface Anchoring Energies of Nematic Liquid Crystals [R] . Wintucky, E. G. 1980

机译：适用于向列相液晶表面锚定能的实验测定的理论模型

Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis

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