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首页> 外文期刊>Journal of bacteriology >A Processive Glycosyltransferase Involved in Glycolipid Synthesis during Phosphate Deprivation in Mesorhizobium loti
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A Processive Glycosyltransferase Involved in Glycolipid Synthesis during Phosphate Deprivation in Mesorhizobium loti

机译:一个过程的糖基转移酶参与糖耐中生根瘤菌的磷酸盐剥夺过程中的糖脂合成。

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Natural habitats are often characterized by a low availability of phosphate. In plants and many bacteria, phosphate deficiency causes different physiological responses, including the replacement of phosphoglycerolipids in the membranes with nonphosphorous lipids. We describe here a processive glycosyltransferase (Pgt) in Mesorhizobium loti (Rhizobiales) involved in the synthesis of di- and triglycosyldiacylglycerols (DGlycD and TGlycD) during phosphate deprivation. Cells of the corresponding Δpgt deletion mutant are deficient in DGlycD and TGlycD. Additional Pgt-independent lipids accumulate in Mesorhizobium after phosphate starvation, including diacylglyceryl trimethylhomoserine (DGTS) and ornithine lipid (OL). The accumulation of the nonphosphorous lipids during phosphate deprivation leads to the reduction of phosphoglycerolipids from 90 to 50%. Nodulation experiments of Mesorhizobium wild type and the Δpgt mutant with its host plant, Lotus japonicus, revealed that DGlycD and TGlycD are not essential for nodulation under phosphate-replete or -deficient conditions. Lipid measurements showed that the Pgt-independent lipids including OL and DGTS accumulate to higher proportions in the Δpgt mutant and therefore might functionally replace DGlycD and TGlycD during phosphate deprivation.
机译:自然栖息地的特点通常是磷酸盐的利用率低。在植物和许多细菌中,磷酸盐缺乏会引起不同的生理反应,包括用无磷脂质替代膜中的磷酸甘油脂。我们在这里描述了间生根瘤菌 Rhizobiales )中的一个过程性糖基转移酶(Pgt),该过程参与了磷酸盐剥夺过程中二糖基和三糖基二酰基甘油(DGlycD和TGlycD)的合成。相应的Δ pgt 缺失突变体的细胞缺乏DGlycD和TGlycD。磷酸盐饥饿后,其他的与Pgt无关的脂质会积聚在根瘤菌中,包括二酰基甘油基三甲基高丝氨酸(DGTS)和鸟氨酸(OL)。磷酸盐剥夺期间非磷脂质的积累导致磷酸甘油脂从90%降低到50%。 中生根瘤菌野生型和Δ pgt 突变体及其寄主植物 Lotus japonicus 的根瘤实验表明,DGlycD和TGlycD对于根瘤不是必需的在磷酸盐充足或不足的条件下。脂质测量显示,包括OL和DGTS在内的Pgt依赖性脂质在Δ pgt 突变体中积累的比例更高,因此在磷酸盐剥夺过程中可能在功能上替代DGlycD和TGlycD。

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