...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >An Atypical Phr Peptide Regulates the Developmental Switch Protein RapH
【24h】

An Atypical Phr Peptide Regulates the Developmental Switch Protein RapH

机译：非典型的Phr肽调节发育开关蛋白RapH。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Under conditions of nutrient limitation and high population density, the bacterium Bacillus subtilis can initiate a variety of developmental pathways. The signaling systems regulating B. subtilis differentiation are tightly controlled by switch proteins called Raps, named after the founding members of the family, which were shown to be response regulator aspartate phosphatases. A phr gene encoding a secreted pentapeptide that regulates the activity of its associated Rap protein was previously identified downstream of 8 of the chromosomally encoded rap genes. We identify and validate here the sequence of an atypical Phr peptide, PhrH, by in vivo and in vitro analyses. Using a luciferase reporter bioassay combined with in vitro experiments, we found that PhrH is a hexapeptide (TDRNTT), in contrast to the other characterized Phr pentapeptides. We also determined that phrH expression is driven by a promoter lying within rapH. Unlike the previously identified dedicated σH-driven phr promoters, it appears that phrH expression most likely requires σA. Furthermore, we show that PhrH can antagonize both of the known activities of RapH: the dephosphorylation of Spo0F and the sequestration of ComA, thus promoting the development of spores and the competent state. Finally, we propose that PhrH is the prototype of a newly identified class of Phr signaling molecules consisting of six amino acids. This class likely includes PhrI, which regulates RapI and the expression, excision, and transfer of the mobile genetic element ICEBs1.
机译：在营养限制和高种群密度的条件下，枯草芽孢杆菌细菌可以引发多种发育途径。枯草芽孢杆菌分化的信号传导系统由名为Raps的开关蛋白严格控制，该开关蛋白以该家族的创始成员命名，其显示为 r 反应调节剂 a spartate p hosphatases。先前在8个染色体编码的 rap 基因的下游发现了一个编码分泌五肽的 phr 基因，该肽调节其相关Rap蛋白的活性。我们通过体内和体外分析鉴定并验证了非典型Phr肽PhrH的序列。使用萤光素酶报告基因生物测定法与体外实验相结合，我们发现PhrH是六肽（TDRNTT），与其他表征的Phr五肽相反。我们还确定 phrH 的表达受 rapH 内的启动子驱动。与先前确定的专用σ H 驱动的 phr 启动子不同， phrH 表达很可能需要σ A 。此外，我们表明PhrH可以拮抗RapH的两种已知活性：Spo0F的去磷酸化和ComA的螯合，从而促进了孢子的发育和感受态。最后，我们提出PhrH是新鉴定的一类由六个氨基酸组成的Phr信号分子的原型。该类可能包括PhrI，它调节RapI以及移动遗传元件ICE Bs 1的表达，切除和转移。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号