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首页> 外文期刊>Journal of bacteriology >Role of the AcsF Protein in Chloroflexus aurantiacus
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Role of the AcsF Protein in Chloroflexus aurantiacus

机译：AcsF蛋白在桔小球藻中的作用

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摘要

The green phototrophic bacteria contain a unique complement of chlorophyll pigments, which self-assemble efficiently into antenna structures known as chlorosomes with little involvement of protein. The few proteins found in chlorosomes have previously been thought to have a primarily structural function. The biosynthetic pathway of the chlorosome pigments, bacteriochlorophylls c, d, and e, is not well understood. In this report, we used spectroscopic, proteomic, and gene expression approaches to investigate the chlorosome proteins of the green filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus. Surprisingly, Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, AcsF, was identified under anaerobic growth conditions. The AcsF protein was found in the isolated chlorosome fractions, and the proteomics analysis suggested that significant portions of the AcsF proteins are not accessible to protease digestion. Additionally, quantitative real-time PCR studies showed that the transcript level of the acsF gene is not lower in anaerobic growth than in semiaerobic growth. Since the proposed enzymatic activity of AcsF requires molecular oxygen, our studies suggest that the roles of AcsF in C. aurantiacus need to be investigated further.
机译：绿色的光养细菌含有独特的叶绿素色素补体，可有效地自组装成天线结构，称为叶绿体，几乎不涉及蛋白质。以前人们曾认为，在脂质体中发现的几种蛋白质具有主要的结构功能。尚未完全了解叶绿体色素，细菌叶绿素 c ， d 和 e 的生物合成途径。在本报告中，我们使用光谱学，蛋白质组学和基因表达方法研究了绿色丝状产氧光养细菌 Chloroflexus aurantiacus 的绿体蛋白。令人惊讶的是，在厌氧生长条件下鉴定了Mg-原卟啉IX单甲酯（氧化）环化酶AcsF。在分离的叶绿体级分中发现了AcsF蛋白，蛋白质组学分析表明，蛋白酶消化无法接近AcsF蛋白的大部分。此外，定量实时PCR研究表明， acsF 基因的转录水平在厌氧生长中并不低于半有氧生长。由于拟议的AcsF的酶活性需要分子氧，因此我们的研究表明AcsF在 C中的作用。需要进一步调查。

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