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首页> 外文期刊>Journal of bacteriology >Thioredoxins in Redox Maintenance and Survival during Oxidative Stress of Bacteroides fragilis
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Thioredoxins in Redox Maintenance and Survival during Oxidative Stress of Bacteroides fragilis

机译：硫氧还蛋白在脆弱拟杆菌的氧化应激过程中的氧化还原维持和存活中。

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The anaerobe Bacteroides fragilis is a gram-negative, opportunistic pathogen that is highly aerotolerant and can persist in aerobic environments for extended periods. In this study, the six B. fragilis thioredoxins (Trxs) were investigated to determine their role during oxidative stress. Phylogenetic analyses of Trx protein sequences indicated that four of the six Trxs (TrxA, TrxC, TrxD, and TrxF) belong to the M-type Trx class but were associated with two different M-type lineages. TrxE and TrxG were most closely associated to Y-type Trxs found primarily in cyanobacteria. Single and multiple trx gene deletions were generated to determine functional differences between the Trxs. The trxA gene was essential, but no anaerobic growth defects were observed for any other single trx deletion or for the ΔtrxC ΔtrxD::cfxA ΔtrxE ΔtrxF ΔtrxG quintuple mutant. Regulation of the trx genes was linked to the oxidative stress response, and all were induced by aerobic conditions. The ΔtrxC ΔtrxE ΔtrxF ΔtrxG and the ΔtrxC ΔtrxD::cfxA ΔtrxE ΔtrxF ΔtrxG multiple deletion strains were impaired during growth in oxidized media, but single trx gene mutants did not have a phenotype in this assay. TrxD was protective during exposure to the thiol oxidant diamide, and expression of trxD was induced by diamide. Diamide-induced expression of trxC, trxE, and trxF increased significantly in a trxD mutant strain, suggesting that there is some capacity for compensation in this complex Trx system. These data provide insight into the role of individual Trxs in the B. fragilis oxidative stress response.
机译：脆弱厌氧杆菌是一种革兰氏阴性，机会性病原体，具有很高的耐氧性，可以在有氧环境中长期存在。在本研究中，六个 B。研究了脆弱类的硫氧还蛋白（Trxs）在氧化应激中的作用。 Trx蛋白序列的系统进化分析表明，六个Trx中的四个（TrxA，TrxC，TrxD和TrxF）属于M型Trx类，但与两个不同的M型谱系相关。 TrxE和TrxG与主要在蓝细菌中发现的Y型Trx密切相关。生成单个和多个 trx 基因缺失，以确定Trx之间的功能差异。 trxA 基因是必不可少的，但对于其他任何单个 trx 缺失或Δ trxC Δ trxD，均未观察到厌氧生长缺陷 :: cfxAΔtrxEΔtrxFΔtrxG五元组突变体。 trx 基因的调节与氧化应激反应有关，所有这些都是由有氧条件诱导的。 Δ trxC Δ trxE Δ trxF Δ trxG 和Δ trxC Δ< em> trxD ::: cfxAΔtrxEΔtrxFΔtrxG在氧化培养基中生长期间，多个缺失菌株受损，但单个 trx 基因突变体在此没有表型分析。 TrxD在暴露于硫醇氧化剂二酰胺的过程中具有保护作用，而 trxD 的表达是由二酰胺诱导的。在 trxD 突变株中，二酰胺诱导的 trxC ， trxE 和 trxF 的表达显着增加。在这种复杂的Trx系统中具有一定的补偿能力。这些数据提供了对单个Trx在 B中的作用的深入了解。脆弱类植物的氧化应激反应。

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