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首页> 外文期刊>Journal of bacteriology >Genomic Island 2 Is an Unstable Genetic Element Contributing to Brucella Lipopolysaccharide Spontaneous Smooth-to-Rough Dissociation
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Genomic Island 2 Is an Unstable Genetic Element Contributing to Brucella Lipopolysaccharide Spontaneous Smooth-to-Rough Dissociation

机译：基因组岛2是不稳定的遗传因素导致布鲁氏菌脂多糖自发平滑到粗糙解离。

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摘要

Brucella is a Gram-negative bacterium that causes a worldwide-distributed zoonosis. The genus includes smooth (S) and rough (R) species that differ in the presence or absence, respectively, of the O-polysaccharide of lipopolysaccharide. In S brucellae, the O-polysaccharide is a critical diagnostic antigen and a virulence determinant. However, S brucellae spontaneously dissociate into R forms, a problem in antigen and S vaccine production. Spontaneous R mutants of Brucella abortus, Brucella melitensis, and Brucella suis carried the chromosomal scar corresponding to genomic island 2 (GI-2) excision, an event causing the loss of the wboA and wboB O-polysaccharide genes, and the predicted excised circular intermediate was identified in B. abortus, B. melitensis, and B. suis cultures. Moreover, disruption of a putative phage integrase gene in B. abortus GI-2 caused a reduction in O-polysaccharide loss rates under conditions promoting S-R dissociation. However, spontaneous R mutants not carrying the GI-2 scar were also detected. These results demonstrate that the phage integrase-related GI-2 excision is a cause of S-R brucella dissociation and that other undescribed mechanisms must also be involved. In the R Brucella species, previous works have shown that Brucella ovis but not Brucella canis lacks GI-2, and a chromosomal scar identical to those in R mutants was observed. These results suggest that the phage integrase-promoted GI-2 excision played a role in B. ovis speciation and are consistent with other evidence, suggesting that this species and B. canis have emerged as two independent lineages.
机译： Brucella 是引起全球分布的人畜共患病的革兰氏阴性细菌。属包括光滑（S）和粗糙（R）种类，它们分别因脂多糖的O-多糖的存在与否而不同。在布鲁氏菌中，O-多糖是关键的诊断抗原和毒性决定因子。然而，布鲁氏菌自发地解离成R形式，这是抗原和S疫苗生产中的问题。 流产布鲁氏菌，布鲁氏菌和 Brucella suis 的自发R突变体携带对应于基因岛2（GI-2）切除的染色体瘢痕，导致 wboA 和 wboB O-多糖基因丢失的事件，并在 B中鉴定出预测的切除的环状中间体。流产， B。 melitensis 和 B。 su 文化。而且，在 B中推定的噬菌体整合酶基因的破坏。在促进S-R解离的条件下，流产 GI-2导致O-多糖损失率降低。但是，还检测到不携带GI-2疤痕的自发性R突变体。这些结果表明，噬菌体整合酶相关的GI-2切除是S-R布氏杆菌解离的原因，还必须涉及其他未描述的机制。在R Brucella 物种中，先前的研究表明， Brucella ovis 但不是 Brucella canis 缺乏GI-2，并且染色体疤痕与那些相同在R突变体中观察到。这些结果表明，噬菌体整合酶促进的GI-2切除在 B中起作用。 ovis 物种，并与其他证据一致，表明该物种与 B。犬已成为两个独立的血统。

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