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首页> 外文期刊>Journal of bacteriology >Manipulating Each MreB of Bdellovibrio bacteriovorus Gives Diverse Morphological and Predatory Phenotypes
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Manipulating Each MreB of Bdellovibrio bacteriovorus Gives Diverse Morphological and Predatory Phenotypes

机译：操纵Bdellovibrio bacteriovorus的每个MreB给出不同的形态和掠夺性表型。

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We studied the two mreB genes, encoding actinlike cytoskeletal elements, in the predatory bacterium Bdellovibrio bacteriovorus. This bacterium enters and replicates within other Gram-negative bacteria by attack-phase Bdellovibrio squeezing through prey outer membrane, residing and growing filamentously in the prey periplasm forming an infective “bdelloplast,” and septating after 4 h, once the prey contents are consumed. This lifestyle brings challenges to the Bdellovibrio cytoskeleton. Both mreB genes were essential for viable predatory growth, but C-terminal green fluorescent protein tagging each separately with monomeric teal-fluorescent protein (mTFP) gave two strains with phenotypic changes at different stages in predatory growth and development. MreB1-mTFP cells arrested growth early in bdelloplast formation, despite successful degradation of prey nucleoid. A large population of stalled bdelloplasts formed in predatory cultures and predation proceeded very slowly. A small proportion of bdelloplasts lysed after several days, liberating MreB1-mTFP attack-phase cells of wild-type morphology; this process was aided by subinhibitory concentrations of an MreB-specific inhibitor, A22. MreB2-mTFP, in contrast, was predatory at an almost wild-type rate but yielded attack-phase cells with diverse morphologies, including spherical, elongated, and branched, the first time such phenotypes have been described. Wild-type predatory rates were seen for all but spherical morphotypes, and septation of elongated morphotypes was achieved by the addition of A22.
机译：我们在掠食性细菌 Bdellovibrio bacteriovorus 中研究了两个编码肌动蛋白样细胞骨架元素的 mreB 基因。该细菌通过侵袭阶段 Bdellovibrio 挤过猎物外膜，进入并在其他革兰氏阴性细菌中复制，并在猎物周质中呈丝状生长并形成感染性“ bdelloplast”，并在4小时后分离。一旦猎物被消耗掉。这种生活方式给 Bdellovibrio 细胞骨架带来了挑战。两个 mreB 基因对于可行的捕食性生长都是必不可少的，但是分别用单体蓝绿色荧光蛋白（mTFP）分别标记的C端绿色荧光蛋白使两种菌株在捕食性生长和发育的不同阶段表现出表型变化。尽管成功降解了猎物核样，但MreB1-mTFP细胞在bdelloplast形成的早期就阻止了生长。在掠食性养殖中形成的大量停滞的原生质体和掠食进行得非常缓慢。几天后，一小部分软化塑料裂解，释放出野生型MreB1-mTFP攻击期细胞。此抑制过程受MreB特异性抑制剂A22亚抑制浓度的影响。相比之下，MreB2-mTFP几乎以野生型的速度被捕食，但是产生的攻击期细胞具有多种形态，包括球形，细长和分支状，这是首次描述此类表型。除球形形态型外，所有野生型都存在掠食性，通过添加A22可以分离出细长形态型。

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