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首页> 外文期刊>Journal of bacteriology >Transcriptional Profiling Analysis of the Global Regulator NorG, a GntR-Like Protein of Staphylococcus aureus
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Transcriptional Profiling Analysis of the Global Regulator NorG, a GntR-Like Protein of Staphylococcus aureus

机译：转录调节分析的全球监管机构NorG，金黄色葡萄球菌的GntR样蛋白。

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The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in resistance to quinolones and β-lactams, such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional-profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. The three putative drug efflux pump genes most positively affected by NorG were the NorB efflux pump (5.1-fold), the MmpL-like protein SACOL2566 (5.2-fold), and the BcrA-like drug transporter SACOL2525 (5.7-fold) genes. The S. aureus predicted MmpL protein showed 53% homology with the MmpL lipid transporter of Mycobacterium tuberculosis, and the putative SACOL2525 protein showed 87% homology with the bacitracin drug transporter BcrA of Staphylococcus hominis. Two pump genes most negatively affected by NorG were the NorC (4-fold) and AbcA (6-fold) genes. Other categories of genes, such as those participating in amino acid, inorganic ion, or nucleotide transporters and metabolism, were also affected by NorG. Real-time reverse transcription (RT)-PCR assays for mgrA, arlS, sarZ, norB, norC, abcA, mmpL, and bcrA-like were carried out to verify microarray data and showed the same level of up- or downregulation by NorG. The norG mutant showed a 2-fold increase in resistance to norfloxacin and rhodamine, both substrates of the NorC transporter, which is consistent with the resistance phenotype conferred by overexpression of norC on a plasmid. These data indicate that NorG has broad regulatory function in S. aureus.
机译：已显示类似GntR的蛋白NorG会影响金黄色葡萄球菌基因，这些基因与喹诺酮和β-内酰胺类的抗性有关，例如编码NorB和AbcA转运蛋白的基因。为了鉴定受NorG调控的靶基因，我们使用金黄色葡萄球菌RN6390及其同基因的 norG :: cat 突变体进行了转录谱分析。我们的数据表明，NorG对全局调节剂 mgrA ， arlsS 和 sarZ 的转录产生积极影响。受NorG影响最大的三个推定药物外排泵基因是NorB外排泵（5.1倍），MmpL样蛋白SACOL2566（5.2倍）和BcrA样转运蛋白SACOL2525（5.7倍）基因。金黄色葡萄球菌预测的MmpL蛋白与结核分枝杆菌的MmpL脂质转运蛋白显示53％的同源性，推定的SACOL2525蛋白与人葡萄球菌的杆菌肽药物转运蛋白BcrA的同源性为87％。受NorG负面影响最大的两个泵基因是NorC（4倍）和AbcA（6倍）基因。其他类别的基因，例如参与氨基酸，无机离子或核苷酸转运蛋白和代谢的基因，也受到NorG的影响。 mgrA ， arlS ， sarZ ， norB ， norC ， abcA ， mmpL 和 bcrA 样的验证，验证了微阵列数据并显示出相同水平的上升-或由NorG下调。 norG 突变体对诺氟沙星和罗丹明的抗性增加了2倍，这是NorC转运蛋白的两个底物，这与 norC 的过量表达所赋予的抗性表型一致。质粒。这些数据表明，NorG在金黄色葡萄球菌中具有广泛的调节功能。

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