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首页> 外文期刊>Journal of bacteriology >Connecting Environment and Genome Plasticity in the Characterization of Transformation-Induced SOS Regulation and Carbon Catabolite Control of the Vibrio cholerae Integron Integrase
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Connecting Environment and Genome Plasticity in the Characterization of Transformation-Induced SOS Regulation and Carbon Catabolite Control of the Vibrio cholerae Integron Integrase

机译：霍乱弧菌整合子整合诱导的SOS调控和碳分解代谢控制的表征中的连接环境和基因组可塑性

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The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed by the activity of the associated integron integrase. Understanding the regulation of integrase expression is pivotal to fully comprehending the role played by this genetic reservoir for bacterial adaptation and its connection with the development of antibiotic resistance. Our previous work established that the integrase is regulated by the bacterial SOS response and that it is induced during bacterial conjugation. Here, we show that transformation, another horizontal gene transfer (HGT) mechanism, also triggers integrase expression through SOS induction, underlining the importance of HGT in genome plasticity. Moreover, we report a new cyclic AMP (cAMP)-cAMP receptor protein (CRP)-dependent regulation mechanism of the integrase, highlighting the influence of the extracellular environment on chromosomal gene content. Altogether, our data suggest an interplay between different stress responses and regulatory pathways for the modulation of the recombinase expression, thus showing how the SI remodeling mechanism is merged into bacterial physiology.
机译：人类病原体霍乱弧菌携带一种染色体超级整合子（SI）。 SI包含数百个串联排列的基因盒，这些基因盒在没有特别的压力施加到细菌的条件下（例如实验室生长期间）稳定。这些盒的重排由相关的整合子整合酶的活性催化。理解整合酶表达的调控对于充分理解这种基因库对细菌的适应及其与抗生素抗性发展的关系至关重要。我们先前的工作确定整合酶受细菌SOS反应的调节，并在细菌结合过程中被诱导。在这里，我们显示了转化，另一种水平基因转移（HGT）机制，也通过SOS诱导触发了整合酶表达，强调了HGT在基因组可塑性中的重要性。此外，我们报告了整合酶的一种新的环状AMP（cAMP）-cAMP受体蛋白（CRP）依赖性调节机制，突出了细胞外环境对染色体基因含量的影响。总而言之，我们的数据表明，不同的应激反应和调节重组酶表达的调节途径之间存在相互作用，从而显示出SI重塑机制是如何融入细菌生理的。

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