...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Expression and Functional Analysis of the Subtilin Immunity Genes spaIFEG in the Subtilin-Sensitive Host Bacillus subtilis MO1099
【24h】

Expression and Functional Analysis of the Subtilin Immunity Genes spaIFEG in the Subtilin-Sensitive Host Bacillus subtilis MO1099

机译：枯草杆菌敏感宿主枯草芽孢杆菌MO1099中枯草杆菌免疫基因spaIFEG的表达和功能分析

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus subtilis ATCC 6633 produces the cationic pore-forming lantibiotic subtilin, which preferentially acts on gram-positive microorganisms; self protection of the producer cells is mediated by the four genes spaIFEG. To elucidate the mechanism of subtilin autoimmunity, we transferred different combinations of subtilin immunity genes under the control of an inducible promoter into the genome of subtilin-sensitive host strain B. subtilis MO1099. Recipient cells acquired subtilin tolerance through expression of either spaI or spaFEG, which shows that subtilin immunity is based on two independently acting systems. Cells coordinately expressing all four immunity genes acquired the strongest subtilin protection level. Quantitative in vivo peptide release assays demonstrated that SpaFEG diminished the quantity of cell-associated subtilin, suggesting that SpaFEG transports subtilin molecules from the membrane into the extracellular space. Homology and secondary structure analyses define SpaFEG as a prototype of lantibiotic immunity transporters that fall into the ABC-2 subfamily of multidrug resistance proteins. Membrane localization of the lipoprotein SpaI and specific interaction of SpaI with the cognate lantibiotic subtilin suggest a function of SpaI as a subtilin-intercepting protein. This interpretation was supported by hexahistidine-mediated 0-? cross-linking between hexahistidine-tagged SpaI and subtilin.
机译：枯草芽孢杆菌 ATCC 6633产生阳离子孔形成羊毛硫抗生素枯草杆菌蛋白酶，其优先作用于革兰氏阳性微生物;生产者细胞的自我保护是由四个基因 spaIFEG 介导的。为了阐明枯草杆菌素自身免疫的机制，我们在诱导型启动子的控制下将枯草杆菌素免疫基因的不同组合转移到了对枯草杆菌素敏感的宿主菌株 B的基因组中。枯草 MO1099。受体细胞通过表达 spaI 或 spaFEG 获得了对枯草蛋白酶的耐受性，这表明枯草蛋白酶的免疫是基于两个独立起作用的系统。协调表达所有四个免疫基因的细胞获得了最强的枯草蛋白酶保护水平。体内定量肽释放测定表明，SpaFEG减少了细胞相关枯草杆菌蛋白酶的量，表明SpaFEG将枯草杆菌蛋白酶分子从膜转运到细胞外空间。同源性和二级结构分析将SpaFEG定义为羊毛硫抗生素免疫转运蛋白的原型，属于多种药物抗性蛋白的ABC-2亚家族。脂蛋白SpaI的膜定位以及SpaI与相关羊毛硫抗生素枯草杆菌蛋白酶的特异性相互作用表明SpaI可以作为一种枯草杆菌蛋白酶拦截蛋白。该解释得到了六组氨酸介导的0-α的支持。六组氨酸标签的SpaI和枯草杆菌蛋白酶之间的交联。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号