...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Distinctive Repertoire of Contingency Genes Conferring Mutation- Based Phase Variation and Combinatorial Expression of Surface Lipoproteins in Mycoplasma capricolum subsp. capricolum of the Mycoplasma mycoides Phylogenetic Cluster
【24h】

Distinctive Repertoire of Contingency Genes Conferring Mutation- Based Phase Variation and Combinatorial Expression of Surface Lipoproteins in Mycoplasma capricolum subsp. capricolum of the Mycoplasma mycoides Phylogenetic Cluster

机译：赋予冠状支原体亚种表面脂蛋白基于突变的相变和组合表达的偶然性基因的独特组成。支原体系统发生簇的心梗

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The generation of surface variation among many divergent species of Mollicutes (mycoplasmas) occurs through stochastic expression patterns of diverse lipoprotein genes. The size and wide distribution of such variable gene sets in minimal (～0.6- to 1.4-Mb) mycoplasmal genomes suggest their key role in the adaptation and survival of these wall-less monoderms. Diversity through variable genes is less clearly established among phylogenetically similar mycoplasmas, such as the Mycoplasma mycoides cluster of ruminant pathogens, which vary widely in host range and pathobiology. Using (i) genome sequences from two members of this clade, Mycoplasma capricolum subsp. capricolum and M. mycoides subsp. mycoides small colony biotype (SC), (ii) antibodies to specific peptide determinants of predicted M. capricolum subsp. capricolum gene products, and (iii) analysis of the membrane-associated proteome of M. capricolum subsp. capricolum, a novel set of six genes (vmcA to vmcF) expressing distinct Vmc (variable M. capricolum subsp. capricolum) lipoproteins is demonstrated. These occur at two separate loci in the M. capricolum subsp. capricolum genome, which shares striking overall similarity and gene synteny with the M. mycoides subsp. mycoides SC genome. Collectively, Vmc expression is noncoordinate and combinatorial, subject to a single-unit insertion/deletion in a 5′ flanking dinucleotide repeat that governs expression of each vmc gene. All vmc genes share modular regions affecting expression and membrane translocation. In contrast, vmcA to vmcD genes at one locus express surface proteins with highly structured size-variable repeating domains, whereas vmcE to vmcF genes express products with short repeats devoid of predicted structure. These genes confer a distinctive, dynamic surface architecture that may represent adaptive differences within this important group of pathogens as well as exploitable diagnostic targets.
机译：在 Mollicutes （支原体）的许多不同物种之间，表面变异的产生是通过多种脂蛋白基因的随机表达模式发生的。这些可变基因集在最小（〜0.6-1.4-Mb）支原体基因组中的大小和广泛分布表明，它们在这些无壁单体的适应和存活中起关键作用。在种系相似的支原体，例如反刍动物病原体 Mycoplasma mycoides 簇之间，通过可变基因的多样性尚不清楚，它们在宿主范围和病理生物学上有很大差异。使用（i）该进化枝的两个成员 Capricolum 亚种的基因组序列。 capricolum 和 M。杀菌剂子空间 mycoides 小菌落生物型（SC），（ii）预测的 M特定肽决定簇的抗体。随心所欲的子空间 capricolum 基因产物，以及（iii） M的膜相关蛋白质组分析。随心所欲的子空间 capricolum ，一组新的六个基因（ vmcA 至 vmcF ）表达不同的Vmc（可变 capricolum 亚种。 capricolum ）脂蛋白被证明。这些发生在 M中的两个单独的基因座上。随心所欲的子空间 capricolum 基因组，与 M具有惊人的整体相似性和基因同义性。杀菌剂子空间 mycoides SC基因组。总的来说，Vmc表达是不协调和组合的，在控制每个 vmc 基因表达的5'侧翼二核苷酸重复序列中受到单个单元的插入/缺失。所有的 vmc 基因共享影响表达和膜易位的模块区域。相反，在一个基因座上的 vmcA 至 vmcD 基因表达具有高度结构化的大小可变重复域的表面蛋白，而 vmcE 至 vmcF 基因表达的产物具有短的重复序列，没有预期的结构。这些基因赋予了独特的动态表面结构，可以代表这一重要病原体以及可利用的诊断靶标之间的适应性差异。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号