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首页> 外文期刊>Journal of bacteriology >Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL
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Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL

机译:小肠结肠炎耶尔森氏菌III型分泌ATPase YscN及其调节​​剂YscL的表征

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Type III secretion is a mechanism used by a broad range of gram-negative bacteria to neutralize eukaryotic defenses by enabling translocation of bacterial proteins directly into the cytoplasm of host cells. The bacterial energy source for secretion is ATP, which is consumed by an ATPase that couples ATP hydrolysis to the unfolding of secreted proteins and the dissociation of their chaperones just prior to secretion. By studying the biochemical properties of YscN and YscL of Yersinia enterocolitica, we have characterized them as the ATPase and ATPase regulator, respectively, of the type III secretion system of this organism. In vivo, YscL and YscN interact with each other, and the overexpression of glutathione S-transferase-YscL abolishes secretion and down-regulates the expression of secretion apparatus components.
机译:III型分泌是广泛的革兰氏阴性细菌通过使细菌蛋白直接转移到宿主细胞的细胞质中和真核生物防御的机制。用于分泌的细菌能源是ATP,ATP消耗该ATP酶,使ATP水解与分泌蛋白的解折叠和伴侣蛋白在分泌前解离有关。通过研究小肠结肠炎耶尔森氏菌的YscN和YscL的生化特性,我们已将它们分别表征为该生物体III型分泌系统的ATPase和ATPase调节剂。在体内,YscL和YscN彼此相互作用,并且谷胱甘肽 S -转移酶-YscL的过表达消除了分泌,并下调了分泌设备成分的表达。

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