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首页> 外文期刊>Journal of bacteriology >Autoregulation Is Essential for Precise Temporal and Steady-State Regulation by the Bordetella BvgAS Phosphorelay
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Autoregulation Is Essential for Precise Temporal and Steady-State Regulation by the Bordetella BvgAS Phosphorelay

机译：自动调节对于Bordetella BvgAS Phosphorelay进行精确的时间和稳态调节至关重要

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摘要

The Bordetella BvgAS virulence control system is prototypical of phosphorelays that use a polydomain sensor and a response regulator to control gene expression in response to environmental cues. BvgAS controls the expression of at least three distinct phenotypic phases (Bvg?, Bvgi, and Bvg+) by differentially regulating the expression of at least four classes of genes. Among the loci regulated by BvgAS is bvgAS itself. We investigated the role of autoregulation in the ability of BvgAS to control multiple gene expression patterns in a temporal and steady-state manner by constructing Bordetella bronchiseptica strains in which the bvgAS promoter was replaced with constitutively active promoters. Our results show that positive autoregulation of bvgAS transcription is required for the temporal expression of multiple phenotypic phases that occurs in response to a shift from Bvg?-phase conditions to Bvg+-phase conditions. Autoregulation was also shown to contribute to steady-state regulation; it influences the sensitivity of the system in response to subtle differences in signal intensity. In addition, considered in relation to BvgA and BvgS activities demonstrated in vitro, our results provide insight into how BvgA and BvgS function mechanistically.
机译： Bordetella BvgAS毒力控制系统是使用多结构域传感器和响应调节剂来控制基因表达以响应环境提示的磷质的原型。 BvgAS通过差异调节at的表达来控制至少三个不同的表型阶段（Bvg ？，Bvg i 和Bvg + ）的表达。至少四类基因。在BvgAS调控的基因座中，有 bvgAS 本身。我们通过构建 bvgAS 启动子为菌株的支气管败血波氏杆菌菌株，研究了自动调节在BvgAS以时间和稳态方式控制多种基因表达模式的能力中的作用。用组成型活性启动子代替。我们的结果表明， bvgAS 转录的正向自动调节对于多个表型阶段的时间表达是必需的，这些表型阶段是响应于从Bvg ？期条件向Bvg + 阶段的条件。自动调节还被证明有助于稳态调节。它会响应信号强度的细微差异而影响系统的灵敏度。此外，考虑到体外证明的BvgA和BvgS活性，我们的结果提供了对BvgA和BvgS如何机械作用的见解。

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