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首页> 外文期刊>Journal of bacteriology >Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum
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Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum

机译：重量克分子渗透压浓度，温度和膜脂质成分调节谷氨酸棒杆菌中甜菜碱转运蛋白BetP的活性。

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The gram-positive soil bacterium Corynebacterium glutamicum, a major amino acid-producing microorganism in biotechnology, is equipped with several osmoregulated uptake systems for compatible solutes, which is relevant for the physiological response to osmotic stress. The most significant carrier, BetP, is instantly activated in response to an increasing cytoplasmic K+ concentration. Importantly, it is also activated by chill stress independent of osmotic stress. We show that the activation of BetP by both osmotic stress and chill stress is altered in C. glutamicum cells grown at and adapted to low temperatures. BetP from cold-adapted cells is less sensitive to osmotic stress. In order to become susceptible for chill activation, cold-adapted cells in addition needed a certain amount of osmotic stimulation, indicating that there is cross talk of these two types of stimuli at the level of BetP activity. We further correlated the change in BetP regulation properties in cells grown at different temperatures to changes in the lipid composition of the plasma membrane. For this purpose, the glycerophospholipidome of C. glutamicum grown at different temperatures was analyzed by mass spectrometry using quantitative multiple precursor ion scanning. The molecular composition of glycerophospholipids was strongly affected by the growth temperature. The modulating influence of membrane lipid composition on BetP function was further corroborated by studying the influence of artificial modulation of membrane dynamics by local anesthetics and the lack of a possible influence of internally accumulated betaine on BetP activity.
机译：革兰氏阳性土壤细菌谷氨酸棒杆菌，是生物技术中主要的氨基酸生产微生物，配备了多种渗透调节的溶质吸收系统，这与对渗透胁迫的生理反应有关。最重要的载体BetP随细胞质K + 浓度的增加而被立即激活。重要的是，它也被与渗透压无关的冷激激活。我们表明，渗透胁迫和寒潮胁迫对BetP的激活在 C中发生了变化。谷氨酸细胞在低温下生长并适应低温。来自冷适应细胞的BetP对渗透压的敏感性较低。为了变得易于激冷，冷适应的细胞还需要一定量的渗透刺激，这表明在BetP活性水平上这两种类型的刺激存在串扰。我们进一步将在不同温度下生长的细胞中BetP调控特性的变化与质膜脂质组成的变化相关联。为此目的，是 C的甘油磷脂脂质体。使用定量多前体离子扫描仪通过质谱分析了在不同温度下生长的谷氨酸。甘油磷脂的分子组成受到生长温度的强烈影响。通过研究局部麻醉药对膜动力学的人工调节的影响以及内部积累的甜菜碱对BetP活性的可能影响的缺乏，进一步证实了膜脂质成分对BetP功能的调节作用。

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