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首页> 外文期刊>Journal of bacteriology >The Escherichia coli Common Pilus and the Bundle-Forming Pilus Act in Concert during the Formation of Localized Adherence by Enteropathogenic E. coli
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The Escherichia coli Common Pilus and the Bundle-Forming Pilus Act in Concert during the Formation of Localized Adherence by Enteropathogenic E. coli

机译：在肠致病性大肠杆菌形成局部粘附过程中，大肠杆菌常见的皮尔和成束的皮尔共同行动

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Although the bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) mediates microcolony formation on epithelial cells, the adherence of BFP-deficient mutants is significantly abrogated, but the mutants are still adherent due to the presence of intimin and possibly other adhesins. In this study we investigated the contribution of the recently described E. coli common pilus (ECP) to the overall adherence properties of EPEC. We found that ECP and BFP structures can be simultaneously observed in the course (between zero time and 7 h during infection) of formation of localized adherence on cultured epithelial cells. These two pilus types colocalized at different levels of the microcolony topology, tethering the adhering bacteria. No evidence of BFP disappearance was found after prolonged infection. When expressed from a plasmid present in nonadherent E. coli HB101, ECP rendered this organism highly adherent at levels comparable to those of HB101 expressing the BFP. Purified ECP bound in a dose-dependent manner to epithelial cells, and the binding was blocked with anti-ECP antibodies, confirming that the pili possess adhesin properties. An ECP mutant showed only a modest reduction in adherence to cultured cells due to background expression levels of BFP and intimin. However, isogenic mutants not expressing EspA or BFP were significantly less adherent when the ecpA gene was also deleted. Furthermore, a ΔespA ΔecpA double mutant (unable to translocate Tir and to establish intimate adhesion) was at least 10-fold less adherent than the ΔespA and ΔecpA single mutants, even in the presence of BFP. A Δbfp ΔespA ΔecpA triple mutant showed the least adherence compared to the wild type and all the isogenic mutant strains tested, suggesting that ECP plays a synergistic role in adherence. Our data indicate that ECP is an accessory factor that, in association with BFP and other adhesins, contributes to the multifactorial complex interaction of EPEC with host epithelial cells.
机译：尽管肠致病性大肠埃希氏菌（EPEC）的成束菌毛（BFP）介导了上皮细胞上的小菌落形成，但BFP缺陷型突变体的粘附被显着消除，但由于内膜蛋白和其他粘附素的存在。在这项研究中，我们调查了最近描述的 E 的贡献。 大肠杆菌常见菌毛（ECP）对EPEC的整体粘附特性的影响。我们发现ECP和BFP结构可以在培养的上皮细胞上形成局部粘附的过程中（感染期间零时间至7小时之间）同时观察到。这两种菌毛类型共同定位在不同的小菌落拓扑结构水平上，从而束缚粘附的细菌。长期感染后未发现BFP消失的迹象。从存在于非粘附性 E中的质粒表达时。大肠埃希氏菌HB101，ECP使该生物具有高度粘附性，其水平与表达BFP的HB101相当。纯化的ECP以剂量依赖的方式与上皮细胞结合，并且该结合被抗ECP抗体阻断，证实菌毛具有粘附素特性。由于BFP和intimin的背景表达水平，ECP突变体对培养细胞的粘附仅表现出适度的降低。然而，当 ecpA 基因也被删除时，不表达EspA或BFP的同基因突变体的附着力明显降低。此外，Δ espA Δ ecpA 双突变体（无法转移Tir和建立紧密的粘附）的粘附程度比Δ espA < / em>和Δ ecpA 单个突变体，即使在存在BFP的情况下也是如此。与野生型和所有同基因突变株相比，Δ bfp Δ espA Δ ecpA 三重突变株的依从性最低。在遵守方面起着协同作用。我们的数据表明，ECP是辅助因子，与BFP和其他粘附素结合，有助于EPEC与宿主上皮细胞的多因素复杂相互作用。

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