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首页> 外文期刊>Journal of bacteriology >Regulation of Superoxide Dismutase (sod) Genes by SarA in Staphylococcus aureus
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Regulation of Superoxide Dismutase (sod) Genes by SarA in Staphylococcus aureus

机译：SarA在金黄色葡萄球菌中对超氧化物歧化酶（sod）基因的调控

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The scavenging of reactive oxygen species (ROS) within cells is regulated by several interacting factors, including transcriptional regulators. Involvement of sarA family genes in the regulation of proteins involved in the scavenging of ROS is largely unknown. In this report, we show that under aerobic conditions, the levels of sodM and sodA transcription, in particular the sodM transcript, are markedly enhanced in the sarA mutant among the tested sarA family mutants. Increased levels of sod expression returned to near the parental level in a single-copy sarA complemented strain. Under microaerophilc conditions, transcription of both sodM and sodA was considerably enhanced in the sarA mutant compared to the wild-type strain. Various genotypic, phenotypic, and DNA binding studies confirmed the involvement of SarA in the regulation of sod transcripts in different strains of Staphylococcus aureus. The sodA mutant was sensitive to an oxidative stress-inducing agent, methyl viologen, but the sarA sodA double mutant was more resistant to the same stressor than the single sodA mutant. These results suggest that overexpression of SodM, which occurs in the sarA background, can rescue the methyl viologen-sensitive phenotype observed in the absence of the sodA gene. Analysis with various oxidative stress-inducing agents indicates that SarA may play a greater role in modulating oxidative stress resistance in S. aureus. This is the first report that demonstrates the direct involvement of a regulatory protein (SarA) in control of sod expression in S. aureus.
机译：细胞内活性氧（ROS）的清除受多种相互作用因子（包括转录调节因子）的调控。 sarA 家族基因在参与清除ROS的蛋白质调控中的参与尚不清楚。在此报告中，我们表明在有氧条件下， sodM 和s odA 转录水平，特别是 sodM 转录水平显着提高被测试的 sarA 家族突变体中的 sarA 突变体中的突变。在单拷贝的 sarA 互补菌株中， sod 表达水平增加，回到亲本水平附近。在微需氧条件下，与野生型菌株相比， sarA 突变体中 sodM 和 sodA 的转录均显着增强。各种基因型，表型和DNA结合研究证实，SarA参与了不同金黄色葡萄球菌菌株中 sod 转录本的调控。 sodA 突变体对氧化应激诱导剂甲基紫精敏感，但是 sarA sodA 双重突变体比单个 sodA对相同的胁迫更具抗性突变体。这些结果表明，发生在 sarA 背景中的SodM的过表达可以挽救在没有 sodA 基因的情况下观察到的对甲基紫精敏感的表型。用各种氧化应激诱导剂进行的分析表明，SarA可能在调节 S的抗氧化应激性中发挥更大的作用。金黄色。这是第一份证明调节蛋白（SarA）直接参与 S中 sod 表达控制的报告。金黄色。

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