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首页> 外文期刊>Journal of bacteriology >The Vibrio cholerae Cpx Envelope Stress Response Senses and Mediates Adaptation to Low Iron
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The Vibrio cholerae Cpx Envelope Stress Response Senses and Mediates Adaptation to Low Iron

机译：霍乱弧菌Cpx信封应力反应感知并介导对低铁的适应

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The Cpx pathway, a two-component system that employs the sensor histidine kinase CpxA and the response regulator CpxR, regulates crucial envelope stress responses across bacterial species and affects antibiotic resistance. To characterize the CpxR regulon in Vibrio cholerae, the transcriptional profile of the pandemic V. cholerae El Tor C6706 strain was examined upon overexpression of cpxR. Our data show that the Cpx regulon of V. cholerae is enriched in genes encoding membrane-localized and transport proteins, including a large number of genes known or predicted to be iron regulated. Activation of the Cpx pathway further led to the expression of TolC, the major outer membrane pore, and of components of two RND efflux systems in V. cholerae. We show that iron chelation, toxic compounds, or deletion of specific RND efflux components leads to Cpx pathway activation. Furthermore, mutations that eliminate the Cpx response or members of its regulon result in growth phenotypes in the presence of these inducers that, together with Cpx pathway activation, are partially suppressed by iron. Cumulatively, our results suggest that a major function of the Cpx response in V. cholerae is to mediate adaptation to envelope perturbations caused by toxic compounds and the depletion of iron.
机译：Cpx途径是一种采用传感器组氨酸激酶CpxA和响应调节剂CpxR的两组分系统，可调节整个细菌物种的关键包膜应激反应并影响抗生素耐药性。为了表征霍乱弧菌中的CpxR调节子，在 cpxR 过度表达后检查了大流行霍乱弧菌El Tor C6706菌株的转录谱。我们的数据表明，霍乱弧菌的Cpx regulon富含编码膜定位和转运蛋白的基因，其中包括大量已知或预期受铁调节的基因。 Cpx途径的激活进一步导致了霍乱弧菌TolC，主要的外膜孔和两个RND外排系统组件的表达。我们表明，铁螯合，有毒化合物或特定RND外排组分的缺失会导致Cpx途径活化。此外，在存在这些诱导物的情况下，消除Cpx反应或其调控子成员的突变会导致生长表型，这些诱导物与Cpx途径激活一起被铁部分抑制。累积地，我们的结果表明霍乱弧菌中Cpx反应的主要功能是介导对有毒化合物和铁耗竭引起的包膜摄动的适应。

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