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首页> 外文期刊>Journal of bacteriology >Nonacetogenic Growth of the Acetogen Acetobacterium woodii on 1,2-Propanediol
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Nonacetogenic Growth of the Acetogen Acetobacterium woodii on 1,2-Propanediol

机译:产乙酸木醋杆菌在1,2-丙二醇上的非产乙酸生长

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Acetogenic bacteria can grow by the oxidation of various substrates coupled to the reduction of CO2 in the Wood-Ljungdahl pathway. Here, we show that growth of the acetogen Acetobacterium woodii on 1,2-propanediol (1,2-PD) as the sole carbon and energy source is independent of acetogenesis. Enzymatic measurements and metabolite analysis revealed that 1,2-PD is dehydrated to propionaldehyde, which is further oxidized to propionyl coenzyme A (propionyl-CoA) with concomitant reduction of NAD. NADH is reoxidized by reducing propionaldehyde to propanol. The potential gene cluster coding for the responsible enzymes includes genes coding for shell proteins of bacterial microcompartments. Electron microscopy revealed the presence of microcompartments as well as storage granules in cells grown on 1,2-PD. Gene clusters coding for the 1,2-PD pathway can be found in other acetogens as well, but the distribution shows no relation to the phylogeny of the organisms.
机译:产乙酸细菌可以通过各种底物的氧化以及Wood-Ljungdahl途径中CO 2 的还原而生长。在这里,我们表明,作为唯一的碳和能源,在1,2-丙二醇(1,2-PD)上的木原乙酸杆菌的生长与产乙酸无关。酶促测量和代谢物分析表明,1,2-PD脱水为丙醛,丙醛进一步被氧化为丙酰辅酶A(丙酰辅酶A),同时降低了NAD。通过将丙醛还原为丙醇将NADH重新氧化。编码负责酶的潜在基因簇包括编码细菌微区室的壳蛋白的基因。电子显微镜显示在1,2-PD上生长的细胞中存在微隔室以及储存颗粒。编码1,2-PD途径的基因簇也可以在其他产乙酸菌中发现,但是其分布与该生物的系统发育无关。

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