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首页> 外文期刊>Journal of bacteriology >Role of O2 in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate
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Role of O2 in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate

机译：O2在豆科根瘤菌生长中的作用。蚕豆和琥珀酸酯的蚕豆3841

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Insertion sequencing (INSeq) analysis of Rhizobium leguminosarum bv. viciae 3841 (Rlv3841) grown on glucose or succinate at both 21% and 1% O2 was used to understand how O2 concentration alters metabolism. Two transcriptional regulators were required for growth on glucose (pRL120207 [eryD] and RL0547 [phoB]), five were required on succinate (pRL100388, RL1641, RL1642, RL3427, and RL4524 [ecfL]), and three were required on 1% O2 (pRL110072, RL0545 [phoU], and RL4042). A novel toxin-antitoxin system was identified that could be important for generation of new plasmidless rhizobial strains. Rlv3841 appears to use the methylglyoxal pathway alongside the Entner-Doudoroff (ED) pathway and tricarboxylic acid (TCA) cycle for optimal growth on glucose. Surprisingly, the ED pathway was required for growth on succinate, suggesting that sugars made by gluconeogenesis must undergo recycling. Altered amino acid metabolism was specifically needed for growth on glucose, including RL2082 (gatB) and pRL120419 (opaA, encoding omega-amino acid:pyruvate transaminase). Growth on succinate specifically required enzymes of nucleobase synthesis, including ribose-phosphate pyrophosphokinase (RL3468 [prs]) and a cytosine deaminase (pRL90208 [codA]). Succinate growth was particularly dependent on cell surface factors, including the PrsD-PrsE type I secretion system and UDP-galactose production. Only RL2393 (glnB, encoding nitrogen regulatory protein PII) was specifically essential for growth on succinate at 1% O2, conditions similar to those experienced by N2-fixing bacteroids. Glutamate synthesis is constitutively activated in glnB mutants, suggesting that consumption of 2-ketoglutarate may increase flux through the TCA cycle, leading to excess reductant that cannot be reoxidized at 1% O2 and cell death.
机译：豆科根瘤菌的插入测序（INSeq）分析。分别以21％和1％的O 2 在葡萄糖或琥珀酸盐上生长的蚕豆蚕豆3841（Rlv3841）用来了解O 2 的浓度如何改变代谢。要在葡萄糖上生长需要两个转录调节子（pRL120207 [ eryD ]和RL0547 [ phoB ]），在琥珀酸盐上需要五个转录调节子（pRL100388，RL1641，RL1642，RL3427和RL4524 [ ecfL ]），而三个则需要1％O 2 （pRL110072，RL0545 [ phoU ]和RL4042）。鉴定了一种新颖的毒素-抗毒素系统，该系统对于产生新的无质粒的根瘤菌菌株可能很重要。 Rlv3841似乎与Entner-Doudoroff（ED）途径和三羧酸（TCA）循环一起使用甲基乙二醛途径，以在葡萄糖上获得最佳生长。出乎意料的是，ED途径是琥珀酸盐生长所必需的，这表明由糖异生作用产生的糖必须经过再循环。在葡萄糖上生长特别需要改变氨基酸代谢，包括RL2082（ gatB ）和pRL120419（ opaA ，编码ω-氨基酸：丙酮酸转氨酶）。在琥珀酸盐上生长特别需要核碱基合成酶，包括核糖磷酸焦磷酸激酶（RL3468 [ prs ]）和胞嘧啶脱氨酶（pRL90208 [ codA ]）。琥珀酸盐的生长特别取决于细胞表面因素，包括PrsD-PrsE I型分泌系统和UDP-半乳糖的产生。只有RL2393（ glnB ，编码氮调节蛋白PII）对于琥珀酸在1％O 2 上的生长特别重要，条件类似于N 2 < / sub>固定类细菌。谷氨酸合成在 glnB 突变体中被组成性激活，这表明2-酮戊二酸的消耗可能会增加整个TCA循环的通量，导致过量的还原剂无法在1％O 2 和细胞死亡。

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