Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli

Karl Skoog; Bill S?derstr?m; Jerker Widengren; Gunnar von Heijne; Daniel O. Daley

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Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli

机译：大肠埃希氏菌细胞分裂过程中的细胞质，然后周质的顺序关闭

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To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

机译：为了可视化活大肠杆菌中细胞分裂的后期阶段，我们对表达细胞质绿色荧光蛋白和周质mCherry的121个细胞进行了光漂白（FRAP）后进行了荧光恢复。我们的数据最终表明，在分裂过程中细胞质先于周质被封闭。

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《Journal of bacteriology》 |2012年第3期|共3页
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Karl Skoog; Bill S?derstr?m; Jerker Widengren; Gunnar von Heijne; Daniel O. Daley;
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2. pH of the Cytoplasm and Periplasm of Escherichia coli: Rapid Measurement by Green Fluorescent Protein Fluorimetry [J] . Jessica C. Wilks, Joan L. Slonczewski Journal of bacteriology . 2007,第15期

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3. Mutations of the Membrane-Bound Disulfide Reductase DsbD That Block Electron Transfer Steps from Cytoplasm to Periplasm in Escherichia coli [J] . Seung-Hyun Cho, Jon Beckwith Journal of bacteriology . 2006,第14期

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4. Overcoming substrate limitations with a cell-free protein synthesis system designed to mimic the Escherichia coli cytoplasm [C] . Michael C. Jewett, James R. Swartz AIChE annual meeting . 2003

机译：通过设计用于模拟大肠杆菌细胞质的无细胞蛋白质合成系统克服底物限制
5. Identification of factors limiting heterologous lipase expression in the cytoplasm and the periplasm as well as display on cell surface of Escherichia coli. [D] . Xu, Yali. 2008

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6. Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli [O] . Karl Skoog, Bill Söderström, Jerker Widengren, 2012

机译：大肠埃希氏菌细胞分裂过程中的细胞质然后周质的顺序关闭
7. Enhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm [O] . Levy Raphael, Ahluwalia Kiran, Bohmann David J., 2013

机译：通过与细胞质中的肽基脯氨酰异构酶FkpA共表达来增强抗体片段分泌到大肠杆菌周质中

Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli

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