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首页> 外文期刊>Journal of bacteriology >The Vibrio cholerae Mannitol Transporter Is Regulated Posttranscriptionally by the MtlS Small Regulatory RNA
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The Vibrio cholerae Mannitol Transporter Is Regulated Posttranscriptionally by the MtlS Small Regulatory RNA

机译：霍乱弧菌甘露醇转运蛋白由MtlS小调节RNA转录后调控。

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摘要

Vibrio cholerae continues to pose a health threat in many developing nations and regions of the world struck by natural disasters. It is a pathogen that rapidly adapts to aquatic environments and the human small intestine. Small regulatory RNAs (sRNAs) may contribute to this adaptability. Specifically, the mannitol operon sRNA (MtlS sRNA; previously designated the IGR7 sRNA) is transcribed antisense to the 5′ untranslated region of the mtl operon, encoding the mannitol-specific phosphotransferase system. Mannitol is a six-carbon sugar alcohol that accumulates in the human small intestine, the primary site of V. cholerae colonization. To better understand the V. cholerae mtl operon at a molecular level, we investigated mtlA expression in the presence of various carbon sources and the role of the MtlS sRNA. We observed that MtlA protein is present only in cells grown on mannitol sugar, whereas MtlS sRNA is expressed during growth on all sugars other than mannitol. In contrast, mtlA mRNA is expressed in similar amounts regardless of the carbon source used for bacterial growth. These observations suggest that the regulation of MtlA protein expression is a posttranscriptional event. We further demonstrate that MtlS sRNA overexpression repressed MtlA synthesis without affecting the stability of the messenger and that this process is largely independent of Hfq. We propose a model in which, when carbon sources other than mannitol are present, MtlS sRNA is transcribed, base pairs with the 5′ untranslated region of the mtlA mRNA, occluding the ribosome binding site, and inhibits the synthesis of the mannitol-specific phosphotransferase system.
机译：霍乱弧菌继续在世界上许多遭受自然灾害的发展中国家和地区构成健康威胁。它是一种迅速适应水生环境和人类小肠的病原体。小型调节性RNA（sRNA）可能有助于这种适应性。具体而言，将甘露醇操纵子sRNA（MtlS sRNA;先前称为IGR7 sRNA）反义转录为 mtl 操纵子的5'非翻译区，编码甘露醇特异性磷酸转移酶系统。甘露醇是六碳糖醇，可在人小肠（霍乱弧菌定殖的主要部位）中积聚。为了在分子水平上更好地了解霍乱弧菌 mtl 操纵子，我们在各种碳源存在下研究了 mtlA 表达以及MtlS sRNA的作用。我们观察到，MtlA蛋白仅存在于甘露醇糖上生长的细胞中，而MtlS sRNA在除甘露醇以外的所有其他糖上的生长过程中均表达。相反， mtlA mRNA的表达量相似，而与细菌生长所用的碳源无关。这些观察表明，MtlA蛋白表达的调节是转录后事件。我们进一步证明，MtlS sRNA过表达抑制MtlA合成，而不影响信使的稳定性，并且该过程在很大程度上与Hfq无关。我们提出了一个模型，其中，当存在除甘露醇以外的碳源时，MtlS sRNA被转录，与 mtlA mRNA的5'非翻译区碱基配对，并阻塞了核糖体结合位点，并抑制了甘露醇特异性磷酸转移酶系统的合成。

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