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首页> 外文期刊>Bulletin of the American Physical Society >APS -70th Annual Meeting of the APS Division of Fluid Dynamics- Event - Continuous Microfluidics (Ecology-on-a-Chip) Experiments for Long Term Observation of Bacteria at Liquid-Liquid Interfaces
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APS -70th Annual Meeting of the APS Division of Fluid Dynamics- Event - Continuous Microfluidics (Ecology-on-a-Chip) Experiments for Long Term Observation of Bacteria at Liquid-Liquid Interfaces

机译：APS-流体动力学APS部门第70届年会-事件-液-液界面上细菌的长期观察的连续微流控（芯片生态学）实验

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摘要

A microfluidic bioassay incorporating a peristaltic pump and chemostat capable of continuously culturing a bacterial suspension through a microchannel for an extended period of time relevant to ecological processes is presented. A single crude oil droplet is dispensed on-chip and subsequently pinned to the top and bottom surfaces of the microchannel to establish a vertical curved oil-water interface to observe bacteria without boundary interference. The accumulation of extracellular polymeric substances (EPS), microbial film formation, and aggregation is provided by DIC microscopy with an EMCCD camera at an interval of 30 sec. Cell-interface interactions such as cell translational and angular motilities as well as encountering, attachment, detachment to the interface are obtained by a high speed camera at 1000 fps with a sampling interval of 10 min. Experiments on extit{Pseudomonas} sp. (P62) and isolated EPS suspensions from extit{Sagitulla Stelleta} and extit{Roseobacter} show rapid formation of bacterial aggregates including EPS streamers stretching tens of drop diameters long. These results provide crucial insights into environmentally relevant processes such as the initiation of marine oil snow, an alternative mode of biodegradation to conventional bioconsumption.
机译：提出了一种微流体生物测定法，其包括蠕动泵和化学恒温器，其能够通过微通道连续培养细菌悬浮液达与生态过程有关的延长时间。将单个原油滴分配到芯片上，然后固定在微通道的顶部和底部表面，以建立垂直的弯曲油水界面，以观察细菌而不受边界干扰。 DIC显微镜用EMCCD相机以30秒的间隔提供细胞外聚合物（EPS）的积累，微生物膜形成和聚集。单元格界面交互（例如单元格平移和角度移动以及与界面的相遇，附着，分离）是通过高速相机以1000 fps的采样间隔10分钟获得的。 extit {Pseudomonas} sp。的实验。 （P62）和来自外部{Sagitulla Stelleta}和外部{Roseobacter}的分离的EPS悬浮液显示了细菌聚集体的快速形成，包括拉伸了数十个液滴直径的EPS流光。这些结果提供了对环境相关过程的关键见解，例如海洋油雪的引发，这是传统生物消费的另一种生物降解方式。

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