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Distribution of petroleum degrading genes and factor analysis of petroleum contaminated soil from the Dagang Oilfield, China

机译:大港油田石油降解基因分布及石油污染土壤的因子分析

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Genes that encode for enzymes that can degrade petroleum hydrocarbons (PHs) are critical for the ability of microorganisms to bioremediate soils contaminated with PHs. Distributions of two petroleum-degrading genes AlkB and Nah in soils collected from three zones of the Dagang Oilfield, Tianjin, China were investigated. Numbers of copies of AlkB ranged between 9.1?×?105 and 1.9?×?107?copies/g dry mass (dm) soil, and were positively correlated with total concentrations of PHs (TPH) (R2?=?0.573, p =?0.032) and alkanes (C33?~?C40) (R2?=?0.914, p Nah gene was distributed relatively evenly among sampling zones, ranging between 1.9?×?107 and 1.1?×?108?copies/g dm soil, and was negatively correlated with concentrations of total aromatic hydrocarbons (TAH) (R2?=??0.567, p =?0.035) and ∑16 PAHs (R2?=??0.599, p =?0.023). Results of a factor analysis showed that individual samples of soils were not ordinated as a function of the zones.
机译:编码可降解石油碳氢化合物(PHs)的酶的基因对于微生物生物修复被PHs污染的土壤的能力至关重要。研究了从天津大港油田三个区域采集的土壤中两个石油降解基因AlkB和Nah的分布。 AlkB的拷贝数在9.1?×?10 5 和1.9?×?10 7 ?拷贝/克土壤干重(dm)之间,并且与kg呈正相关。 PHs(TPH)(R 2 ?=?0.573,p =?0.032)和烷烃(C33?〜?C40)(R 2 ?== 0.914)的总浓度,p Nah基因在采样区之间相对均匀分布,范围为1.9?×?10 7 和1.1?×?10 8 ?copies / g dm土壤。与总芳香烃(TAH)(R 2 ?=?0.567,p =?0.035)和∑16 PAHs(R 2 ?=? 0.599,p =?0.023)。因子分析的结果表明,土壤的单个样本并未随区域而变化。

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