...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Scientific reports. >An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
【24h】

An improved agar-plate method for studying root growth and response of Arabidopsis thaliana

机译：一种改进的琼脂平板法研究拟南芥根系生长和响应的方法

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Arabidopsis thaliana is a widely used model plant for plant biology research. Under traditional agar-plate culture system (TPG, traditional plant-growing), both plant shoots and roots are exposed to illumination, and roots are grown in sucrose-added medium. This is not a natural environment for the roots and may cause artifact responses. We have developed an improved agar-plate culture system (IPG, improved plant-growing) where shoots are illuminated but roots are grown in darkness without sucrose addition. Compared to TPG, IPG produced plants with significantly less total root length, lateral root length and root hair density, although their primary roots were longer. Root gravitropism, PIN2 (an auxin efflux carrier) abundance, H+ efflux or Ca2+ influx in root apexes, were weaker in IPG-grown roots than those in TPG-grown roots. We conclude that IPG offers a more natural way to study the root growth and response of Arabidopsis thaliana .
机译：拟南芥是一种广泛用于植物生物学研究的模型植物。在传统的琼脂平板培养系统（TPG，传统植物生长）下，植物的芽和根都暴露在光照下，并且根在添加蔗糖的培养基中生长。对于根部而言，这不是自然环境，并且可能导致伪影响应。我们已经开发了一种改良的琼脂平板培养系统（IPG，改进了植物的生长），在这种系统中，可以照亮枝条，但是根在黑暗中生长而无需添加蔗糖。与TPG相比，IPG产生的植物的总根长，侧根长和根毛密度显着降低，尽管它们的初生根更长。根尖的向地性，PIN2（一种生长素外排载体）的丰度，H + 外排或Ca 2 + 流入根尖，在IPG生长的根中比TPG-生长的根。我们得出结论，IPG为研究拟南芥的根系生长和响应提供了一种更自然的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号